Department of Gastroenterology, Trinity Academic Gastroenterology Group Research Centre, Trinity Centre, Tallaght University Hospital, Dublin 24, Ireland.
School of Medicine, Trinity College Dublin, Dublin, Ireland.
Methods Mol Biol. 2021;2283:37-43. doi: 10.1007/978-1-0716-1302-3_5.
Helicobacter pylori infection can be detected on endoscopic biopsy of the gastric mucosa, by means of several techniques. The biopsy specimens are usually taken from the prepyloric region, but additional biopsy specimens obtained proximally increase the sensitivity of invasive tests and are recommended, especially if the patient has recently been treated with a proton-pump inhibitor. The effects of an increased risk of sampling error and the lower prevalence of H. pylori infection on the diagnostic accuracy of standard invasive tests needs to be considered. Despite evidence of enhanced yield with additional biopsies, combined Rapid Urease Tests (RUTs) have not been widely adopted. The other endoscopic tests, histology , and culture are also prone to sampling error and adoption of appropriate biopsy protocols should be widely adopted to enhance diagnostic yield.
幽门螺杆菌感染可通过内镜胃黏膜活检，采用多种技术检测。活检标本通常取自胃前区，但近端附加活检标本可增加侵袭性检查的敏感性，因此推荐进行，尤其是患者近期使用质子泵抑制剂治疗的情况下。需要考虑增加采样误差风险和幽门螺杆菌感染较低患病率对标准侵袭性检查诊断准确性的影响。尽管有证据表明附加活检可提高检出率，但联合快速尿素酶试验（RUT）并未广泛应用。其他内镜检查、组织学和培养也容易出现采样误差，应广泛采用适当的活检方案以提高诊断检出率。
