幽门螺杆菌毒力因子基因分型。

Helicobacter pylori Virulence Factor Genotyping.

机构信息

School of Medicine, Trinity College Dublin, Dublin, Ireland.

Foodborne and Waterborne Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

出版信息

Methods Mol Biol. 2021;2283:93-106. doi: 10.1007/978-1-0716-1302-3_11.

DOI:10.1007/978-1-0716-1302-3_11

PMID:33765313

Abstract

Helicobacter pylori (H. pylori) infection causes chronic gastritis, peptic ulcers, gastric adenocarcinoma, and mucosa-associated lymphoid tissue (MALT) lymphoma. Bacterial, host, and environmental factors influence the progression of disease from superficial gastritis to cancer. H. pylori is genetically diverse, and expression of its specific virulence factors has been linked to increased risk of more severe pathologies. Described in this chapter is a protocol for detecting important H. pylori virulence factors by firstly extracting DNA from culture material or stomach tissue biopsies, followed by PCR amplification and agarose gel electrophoresis.

摘要

幽门螺杆菌（H. pylori）感染可导致慢性胃炎、消化性溃疡、胃癌和黏膜相关淋巴组织（MALT）淋巴瘤。细菌、宿主和环境因素影响疾病从浅表性胃炎向癌症的进展。H. pylori 具有遗传多样性，其特定毒力因子的表达与更严重病理的风险增加有关。本章描述了一种从培养物或胃组织活检中提取 DNA，然后进行 PCR 扩增和琼脂糖凝胶电泳检测重要 H. pylori 毒力因子的方法。