大鼠急性运动后，骨骼肌膜胆固醇含量降低对于增加胰岛素刺激的葡萄糖摄取并非必需。
Reduced membrane cholesterol content in skeletal muscle is not essential for greater insulin-stimulated glucose uptake after acute exercise by rats.
机构信息
Muscle Biology Laboratory, School of Kinesiology, University of Michigan, Ann Arbor, MI 48109-1048, USA.
出版信息
Appl Physiol Nutr Metab. 2021 Jun;46(6):685-689. doi: 10.1139/apnm-2021-0130. Epub 2021 Mar 25.
Abstract
One exercise session can elevate insulin-stimulated glucose uptake (GU) by skeletal muscle, but it is uncertain if this effect is accompanied by altered membrane cholesterol content, which is reportedly inversely related to insulin-stimulated GU. Muscles from sedentary (SED) or exercised 3 h post-exercise (3hPEX) rats were evaluated for GU, membrane cholesterol, and phosphorylation of cholesterol regulatory proteins (pHMCGR and pABCA1). Insulin-stimulated GU for 3hPEX exceeded SED. Membrane cholesterol, pHMCGR and pABCA1 did not differ between groups. Alterations in membrane cholesterol and phosphorylation of proteins that regulate muscle cholesterol are not essential for elevated insulin-stimulated GU in skeletal muscle after acute exercise.
摘要
一次运动即可提高骨骼肌的胰岛素刺激葡萄糖摄取（GU），但尚不清楚这种作用是否伴有膜胆固醇含量的改变，据报道，后者与胰岛素刺激的 GU 呈负相关。评估了久坐（SED）或运动后 3 小时（3hPEX）大鼠的肌肉的 GU、膜胆固醇和胆固醇调节蛋白的磷酸化（pHMCGR 和 pABCA1）。3hPEX 的胰岛素刺激 GU 超过 SED。两组之间的膜胆固醇、pHMCGR 和 pABCA1 没有差异。急性运动后，骨骼肌中胰岛素刺激 GU 升高时，膜胆固醇和调节肌肉胆固醇的蛋白质磷酸化的改变并非必需。
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