Post Graduate Department of Biotechnology, St. Xavier's College (Autonomous), 30, Mother Teresa Sarani, Kolkata 700016, West Bengal, India.
Post Graduate Department of Biotechnology, St. Xavier's College (Autonomous), 30, Mother Teresa Sarani, Kolkata 700016, West Bengal, India.
Ecotoxicol Environ Saf. 2021 Jun 1;215:112055. doi: 10.1016/j.ecoenv.2021.112055. Epub 2021 Mar 23.
The present manuscript elucidated the ameliorative potential of nano-maghemite (FeNPs) against the hazardous effects of fluoride toxicity in the sensitive rice cultivar, IR-64. Fluoride pollution triggered bioaccumulation in root, shoot and spikelets which inhibited reproduction, agronomic development and mineral uptake. Suppressed activity of enzymatic antioxidants and excessive cobalt translocation manifested severe ROS-induced oxidative injuries. Seedling priming with FeNPs reduced fluoride bioaccumulation and promoted efficient uptake of macroelements and micronutrients like potassium, calcium, iron, zinc, copper, nickel, manganese, selenium and vanadium and reduced the translocation of cobalt in mature seedlings during stress. This altogether triggered growth and activated the enzymes like SOD, CAT, APX and GPOX. High accumulation of non-enzymatic antioxidants like proline, anthocyanins, flavonoids, phenolics along with stimulated GSH synthesis (determined from high GR, GST and GPX activity) and glyoxalase activity enabled FeNP-pulsed plants to efficiently scavenge ROS, O, HO and methylglyoxal, and mitigate oxidative injuries. The ROS production was also lowered due to suppressed NADPH oxidase activity. This ensured subsequent revitalization of Hill activity and the level of photosynthetic pigments. Due to reduced fluoride partitioning and improved nutritional sink, the grain and panicle development in FeNP-primed, stressed seedlings were more stimulated than even control sets. Overall, our findings supported by statistical modelling established the potential of iron-nanotechnology in promoting safe rice cultivation even in fluoride-polluted environments.
本手稿阐明了纳米磁赤铁矿（FeNPs）对敏感水稻品种 IR-64 中氟毒性危害的改善潜力。氟污染引发了根、茎和小穗中的生物累积，从而抑制了繁殖、农艺发育和矿物质吸收。酶抗氧化剂活性的抑制和过量钴的转移表现出严重的 ROS 诱导的氧化损伤。用 FeNPs 对幼苗进行浸种处理可减少氟的生物累积，促进钾、钙、铁、锌、铜、镍、锰、硒和钒等大量元素和微量元素的有效吸收，并减少成熟幼苗在胁迫期间钴的转移。这一切共同引发了生长，并激活了 SOD、CAT、APX 和 GPOX 等酶。脯氨酸、花青素、类黄酮、酚类等非酶抗氧化剂的大量积累，以及 GSH 合成的刺激（通过高 GR、GST 和 GPX 活性确定）和糖醛酸酶活性，使 FeNP 脉冲处理的植物能够有效地清除 ROS、O、HO 和甲基乙二醛，并减轻氧化损伤。由于 NADPH 氧化酶活性的抑制，ROS 的产生也降低了。这确保了随后的希尔活性和光合色素水平的恢复。由于氟的分配减少和营养吸收增加，与对照组相比，FeNP 预处理、胁迫下的幼苗的籽粒和穗发育受到了更大的刺激。总的来说，我们的研究结果支持了铁纳米技术在促进安全水稻种植方面的潜力，即使在氟污染的环境中也是如此。
