控制电子剂量以改善对敏感样品的低剂量成像的指南。

Guide to controlling the electron dose to improve low-dose imaging of sensitive samples.

机构信息

Electron Microscopy Laboratory, Wroclaw University of Science and Technology, Wybrzeze Wyspianskiego 27, 50370, Wroclaw, Poland; Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Weigla 12, 53114 Wroclaw, Poland.

出版信息

Micron. 2021 Jun;145:103058. doi: 10.1016/j.micron.2021.103058. Epub 2021 Mar 18.

DOI:10.1016/j.micron.2021.103058

PMID:33765604

Abstract

This work describes the methods of beam current measurement and proposes the construction of an easily fabricated Faraday cup for use in any side-entry transmission electron microscope. Moreover, a method of calculating the dose of electrons with the use of a digital camera is proposed and verified with the use of two slightly different microscope setups. The method turned out to be particularly useful for estimating the smallest doses of electrons used in the imaging of particularly sensitive samples.

摘要

本工作描述了束流测量的方法，并提出了一种易于制造的用于任何侧入射透射电子显微镜的法拉第杯的结构。此外，还提出了一种使用数字相机计算电子剂量的方法，并使用两种略有不同的显微镜设置进行了验证。该方法对于估计用于对特别敏感的样品成像的最小电子剂量特别有用。