MOE Joint International Research Laboratory of Animal Health and Food Safety, Nanjing Agricultural University, Nanjing 210095, PR China; Shanghai Key Laboratory of Molecular Imaging, Shanghai University of Medicine and Health Sciences, Shanghai 201318, China.
MOE Joint International Research Laboratory of Animal Health and Food Safety, Nanjing Agricultural University, Nanjing 210095, PR China.
Res Vet Sci. 2021 May;136:336-342. doi: 10.1016/j.rvsc.2021.03.017. Epub 2021 Mar 18.
In pregnant sows, heat stress (HS) not only affects sows, but also has long-term effects on offspring growth. However, it is still unclear how HS in pregnant sows influences offspring skeletal muscle development. In this study, 12 sows with similar body conditions were assigned into either a control (CON) or an HS group. The CON sows were housed at 18-22 ℃, and the sows in the HS group were housed at 28-32 ℃ from day 85 to 114 of pregnancy. The results showed that maternal HS decreased the total protein content (P < 0.05) and prolactin level (P < 0.05), yet increased the triglyceride content (P < 0.05) of milk. The piglets of both groups had similar body weight and longissimus dorsi (LD) muscle weight at birth, but body weight (P < 0.05) and LD weight (P < 0.05) was significantly lower at weaning age in the HS group. Increased expression of myostatin (MSTN) (P < 0.05) and its receptor (P < 0.05) in the LD of HS piglets was observed at weaning. The following decreased in HS piglets: expression of serine/threonine-specific protein kinase (P < 0.05), the mammalian target of rapamycin (P < 0.05), and glycogen synthase kinase-3β (P < 0.05) signal pathway-involved proteins. The results indicated that maternal HS during late pregnancy influenced offspring LD muscle growth via the activated MSTN pathway. This effect may be related to sow's milk composition.
在妊娠母猪中，热应激（HS）不仅会影响母猪，而且对后代的生长也会产生长期影响。然而，妊娠母猪中的 HS 如何影响后代骨骼肌发育仍不清楚。在这项研究中，将 12 头体况相似的母猪分为对照组（CON）或 HS 组。CON 组母猪的饲养温度为 18-22℃，HS 组母猪从妊娠第 85 天到 114 天的饲养温度为 28-32℃。结果表明，母体 HS 降低了总蛋白含量（P<0.05）和催乳素水平（P<0.05），但增加了乳中的甘油三酯含量（P<0.05）。两组仔猪出生时体重和背最长肌（LD）肌肉重量相似，但 HS 组仔猪在断奶时体重（P<0.05）和 LD 重量（P<0.05）显著降低。在断奶时，HS 仔猪的肌肉生长抑制素（MSTN）（P<0.05）及其受体（P<0.05）在 LD 中的表达增加。HS 仔猪中以下蛋白的表达减少：丝氨酸/苏氨酸特异性蛋白激酶（P<0.05）、雷帕霉素靶蛋白（P<0.05）和糖原合成酶激酶-3β（P<0.05）信号通路相关蛋白。结果表明，妊娠后期母体 HS 通过激活 MSTN 途径影响后代 LD 肌肉生长。这种影响可能与母猪乳汁的成分有关。
