Zhong Jiale, Zhen Wenrui, Bai Dongying, Hu Xiaodi, Zhang Haojie, Zhang Ruilin, Ito Koichi, Zhang Yi, Zhang Bingkun, Ma Yanbo
Department of Animal Physiology, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471000, China.
Henan International Joint Laboratory of Animal Welfare and Health Breeding, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471000, China.
Antioxidants (Basel). 2024 Mar 13;13(3):341. doi: 10.3390/antiox13030341.
The aim of this study was to investigate the effects of aspirin eugenol ester (AEE) on liver oxidative damage and energy metabolism in immune-stressed broilers. In total, 312 broilers were divided into 4 groups (saline, LPS, SAEE, and LAEE). Broilers in the saline and LPS groups were fed a basal diet; the SAEE and LAEE groups had an added 0.01% AEE in their diet. Broilers in the LPS and LAEE groups were injected with lipopolysaccharides, while the saline and SAEE groups were injected with saline. Results showed that AEE increased the body weight, average daily gain, and average daily feed intake, as well as decreasing the feed conversion ratio of immune-stressed broilers. AEE protects against oxidative damage in immune-stressed broiler livers by elevating the total antioxidant capacity, superoxide dismutase activity, and glutathione S-transferase alpha 3 () and glutaredoxin 2 () expression, while decreasing malondialdehyde content. AEE lessened inflammation by reducing prostaglandin-F2α production and prostaglandin-endoperoxide synthase 2 () and interleukin-1beta () expression. AEE decreased oxidative phosphorylation rates by increasing succinic acid levels and lowering both adenosine diphosphate (ADP) levels and ceroid lipofuscinosis neuronal 5 () expression. AEE modulated the metabolism of phenylalanine, tyrosine, lipids, and cholesterol by reducing the phenyllactate and L-arogenate levels, lowering dopachrome tautomerase () and apolipoprotein A4 () expression, and increasing phenylpyruvic acid and dopa decarboxylase () expression. In summary, AEE can effectively alleviate liver oxidative damage and energy metabolism disorders in immune-stressed broilers.
本研究旨在探讨阿司匹林丁香酚酯（AEE）对免疫应激肉鸡肝脏氧化损伤和能量代谢的影响。总共312只肉鸡被分为4组（生理盐水组、脂多糖组、低剂量AEE组和高剂量AEE组）。生理盐水组和脂多糖组的肉鸡饲喂基础日粮；低剂量AEE组和高剂量AEE组的日粮中添加0.01%的AEE。脂多糖组和高剂量AEE组的肉鸡注射脂多糖，而生理盐水组和低剂量AEE组注射生理盐水。结果表明，AEE增加了免疫应激肉鸡的体重、平均日增重和平均日采食量，同时降低了饲料转化率。AEE通过提高总抗氧化能力、超氧化物歧化酶活性、谷胱甘肽S-转移酶α3（）和谷氧还蛋白2（）的表达，同时降低丙二醛含量，保护免疫应激肉鸡肝脏免受氧化损伤。AEE通过减少前列腺素-F2α的产生以及前列腺素内过氧化物合酶2（）和白细胞介素-1β（）的表达来减轻炎症。AEE通过增加琥珀酸水平、降低二磷酸腺苷（ADP）水平和类脂褐质神经元5（）的表达来降低氧化磷酸化速率。AEE通过降低苯乳酸和L-阿糖酸水平、降低多巴色素互变异构酶（）和载脂蛋白A4（）的表达以及增加苯丙酮酸和多巴脱羧酶（）的表达来调节苯丙氨酸、酪氨酸、脂质和胆固醇的代谢。总之，AEE可以有效缓解免疫应激肉鸡的肝脏氧化损伤和能量代谢紊乱。
