大鼠微栓塞模型中缺氧区域的恢复。
Recovery of Hypoxic Regions in a Rat Model of Microembolism.
机构信息
Amsterdam UMC, University of Amsterdam, Biomedical Engineering and Physics, Amsterdam Cardiovascular Sciences, Amsterdam, the Netherlands.
Amsterdam UMC, University of Amsterdam, Biomedical Engineering and Physics, Amsterdam Cardiovascular Sciences, Amsterdam, the Netherlands.
出版信息
J Stroke Cerebrovasc Dis. 2021 Jun;30(6):105739. doi: 10.1016/j.jstrokecerebrovasdis.2021.105739. Epub 2021 Mar 23.
OBJECTIVES
Endovascular treatment (EVT) has become the standard of care for acute ischemic stroke. Despite successful recanalization, a limited subset of patients benefits from the new treatment. Human MRI studies have shown that during removal of the thrombus, a shower of microclots is released from the initial thrombus, possibly causing new ischemic lesions. The aim of the current study is to quantify tissue damage following microembolism.
MATERIALS AND METHODS
In a rat model, microembolism was generated by injection of a mixture of polystyrene fluorescent microspheres (15, 25 and 50 µm in diameter). The animals were killed at three time-points: day 1, 3 or 7. AMIRA and IMARIS software was used for 3D reconstruction of brain structure and damage, respectively.
CONCLUSIONS
Microembolism induces ischemia, hypoxia and infarction. Infarcted areas persist, but hypoxic regions recover over time suggesting that repair processes in the brain rescue the regions at risk.
目的
血管内治疗（EVT）已成为急性缺血性脑卒中的标准治疗方法。尽管血管再通成功，但只有一部分患者从新的治疗中获益。人类 MRI 研究表明，在血栓清除过程中，初始血栓会释放出一阵微栓子，可能导致新的缺血性损伤。本研究旨在定量分析微栓塞后的组织损伤。
材料和方法
在大鼠模型中，通过注射聚苯乙烯荧光微球（直径为 15、25 和 50 µm）的混合物来产生微栓塞。动物在三个时间点（第 1、3 或 7 天）处死。使用 AMIRA 和 IMARIS 软件分别进行脑结构和损伤的 3D 重建。
结论
微栓塞会引起缺血、缺氧和梗死。梗死区域持续存在，但缺氧区域随着时间的推移会恢复，这表明大脑中的修复过程可以挽救处于危险中的区域。
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