微小 RNA-378a-3p 下调作为宫颈癌不良临床结局的新型生物标志物。

MicroRNA-378a-3p Downregulation as a Novel Biomarker with Poor Clinical Outcomes in Cervical Cancer.

机构信息

Department of Clinical Laboratory Medicine, Beijing Hospital of Integrated Chinese and Western Medicine, Beijing 100039, China.

出版信息

Biomed Environ Sci. 2021 Mar 20;34(3):213-221. doi: 10.3967/bes2021.026.

DOI:10.3967/bes2021.026

PMID:33766217

Abstract

OBJECTIVE

Cervical cancer (CC) is one of the most common malignant tumors in gynecology. This study aimed to investigate the prognostic significance of serum microRNA (miR)-378a-3p in CC and the effect of miR-378a-3p on tumor growth.

METHODS

Real-time quantitative polymerase chain reaction analysis was used to measure the expression of miR-378a-3p in serum from patients with CC and healthy control subjects as well as from CC tissues and adjacent normal tissues. The association between serum miR-378a-3p levels and clinicopathological factors was analyzed. The correlation between miR-378a-3p levels and overall survival (OS) of CC patients was determined by Kaplan-Meier analysis. The CC cell proliferation and migration abilities after transfection of miR-378a-3p mimics were detected by Cell Counting Kit-8 and scratch wound healing assays, respectively. Tumor volume and weight in mice treated with miR-378a-3p were measured using a caliper and an electronic balance.

RESULTS

MiR-378a-3p expression was downregulated in the serum and tissues of CC patients compared to that in healthy control subjects and normal tissues, respectively. Low expression of miR-378a-3p was positively correlated with large tumor size, advanced tumor stage, and lymph node metastasis. The OS of patients with low expression of miR-378a-3p was significantly lower than that of patients with high expression. Overexpression of miR-378a-3p suppressed the proliferation and migration of CC cells. studies indicated that overexpression of miR-378a-3p was associated with decreased tumor volume and weight in mice.

CONCLUSION

MiR-378a-3p downregulation is associated with the development and prognosis of CC, suggesting that it may be a potential biomarker for CC.

摘要

目的

宫颈癌（CC）是妇科最常见的恶性肿瘤之一。本研究旨在探讨血清 microRNA（miR）-378a-3p 在 CC 中的预后意义及其对肿瘤生长的影响。

方法

实时定量聚合酶链反应分析用于测量 CC 患者和健康对照者血清以及 CC 组织和相邻正常组织中 miR-378a-3p 的表达。分析血清 miR-378a-3p 水平与临床病理因素的关系。通过 Kaplan-Meier 分析确定 miR-378a-3p 水平与 CC 患者总生存期（OS）的相关性。通过细胞计数试剂盒-8 和划痕愈合试验分别检测转染 miR-378a-3p 模拟物后 CC 细胞增殖和迁移能力。使用卡尺和电子天平测量 miR-378a-3p 处理的小鼠的肿瘤体积和重量。

结果

与健康对照者和正常组织相比，CC 患者血清和组织中 miR-378a-3p 的表达下调。miR-378a-3p 低表达与肿瘤体积大、肿瘤分期晚和淋巴结转移有关。miR-378a-3p 低表达患者的 OS 明显低于 miR-378a-3p 高表达患者。过表达 miR-378a-3p 抑制 CC 细胞的增殖和迁移。研究表明，miR-378a-3p 过表达与小鼠肿瘤体积和重量的降低有关。