Department of Clinical Laboratory Medicine, Beijing Hospital of Integrated Chinese and Western Medicine, Beijing 100039, China.
Biomed Environ Sci. 2021 Mar 20;34(3):213-221. doi: 10.3967/bes2021.026.
Cervical cancer (CC) is one of the most common malignant tumors in gynecology. This study aimed to investigate the prognostic significance of serum microRNA (miR)-378a-3p in CC and the effect of miR-378a-3p on tumor growth.
Real-time quantitative polymerase chain reaction analysis was used to measure the expression of miR-378a-3p in serum from patients with CC and healthy control subjects as well as from CC tissues and adjacent normal tissues. The association between serum miR-378a-3p levels and clinicopathological factors was analyzed. The correlation between miR-378a-3p levels and overall survival (OS) of CC patients was determined by Kaplan-Meier analysis. The CC cell proliferation and migration abilities after transfection of miR-378a-3p mimics were detected by Cell Counting Kit-8 and scratch wound healing assays, respectively. Tumor volume and weight in mice treated with miR-378a-3p were measured using a caliper and an electronic balance.
MiR-378a-3p expression was downregulated in the serum and tissues of CC patients compared to that in healthy control subjects and normal tissues, respectively. Low expression of miR-378a-3p was positively correlated with large tumor size, advanced tumor stage, and lymph node metastasis. The OS of patients with low expression of miR-378a-3p was significantly lower than that of patients with high expression. Overexpression of miR-378a-3p suppressed the proliferation and migration of CC cells. studies indicated that overexpression of miR-378a-3p was associated with decreased tumor volume and weight in mice.
MiR-378a-3p downregulation is associated with the development and prognosis of CC, suggesting that it may be a potential biomarker for CC.
宫颈癌（CC）是妇科最常见的恶性肿瘤之一。本研究旨在探讨血清 microRNA（miR）-378a-3p 在 CC 中的预后意义及其对肿瘤生长的影响。
实时定量聚合酶链反应分析用于测量 CC 患者和健康对照者血清以及 CC 组织和相邻正常组织中 miR-378a-3p 的表达。分析血清 miR-378a-3p 水平与临床病理因素的关系。通过 Kaplan-Meier 分析确定 miR-378a-3p 水平与 CC 患者总生存期（OS）的相关性。通过细胞计数试剂盒-8 和划痕愈合试验分别检测转染 miR-378a-3p 模拟物后 CC 细胞增殖和迁移能力。使用卡尺和电子天平测量 miR-378a-3p 处理的小鼠的肿瘤体积和重量。
与健康对照者和正常组织相比，CC 患者血清和组织中 miR-378a-3p 的表达下调。miR-378a-3p 低表达与肿瘤体积大、肿瘤分期晚和淋巴结转移有关。miR-378a-3p 低表达患者的 OS 明显低于 miR-378a-3p 高表达患者。过表达 miR-378a-3p 抑制 CC 细胞的增殖和迁移。研究表明，miR-378a-3p 过表达与小鼠肿瘤体积和重量的降低有关。
miR-378a-3p 的下调与 CC 的发生和预后有关，表明它可能是 CC 的潜在生物标志物。
