Ma Guilan, Tan Yan, Tian Yaling, Bao Lunmin, Jiang Hongmei
Department of Clinical Microbiology and Immunology, School of Medical Laboratory Science, Guizhou Medical University, Guiyang 550025, China.
Department of Laboratory Medicine, Tianyou Hospital Affiliated to Wuhan University of Science and Technology, Wuhan 430000, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2021 Mar;37(3):193-198.
Objective To investigate the effects of tolerogenic dendritic cells (tolDCs) induced by nuclear factor κB oligodeoxynucleotide decoy (NF-κB ODN decoy) on Th1 cells, Th2 cells, Th17 cells and regulatory T cells (Tregs) and the intervention effects on collagen-induced arthritis (CIA) rats. Methods SD female rats used to establish CIA rat models were divided into four groups, including a CIA model group, a bovine type II collagen-decoy-dendritic cell (Col2-decoy DC) treatment group, a blank control group, and a Col2-decoy DC control group. On the 20th days after the first immunization, the rats were injected with tolDCs via the tail vein, and the rats were sacrificed on the 7th weeks. The proportions of Th1 cells, Th2 cells, Th17 cells, and Tregs in the rat spleen were detected by flow cytometry. The ankle joint pathomorphological change was evaluated by HE staining, and the arthritis index (AI) was scored. Results Compared with the CIA model group, the Col2-decoy DC group had lower AI and milder ankle joint pathomorphological change. The percentages of Th1 cells and Th17 cells in the spleen CD4 T cells decreased, while the percentages of Th2 cells and Tregs increased. Conclusion The treatment of tolDCs can alleviate the inflammation and arthropathy of CIA rats by reducing the proportion of Th1 and Th17 cells in CD4 T cells.
目的 探讨核因子κB寡脱氧核苷酸诱饵（NF-κB ODN诱饵）诱导的耐受性树突状细胞（tolDCs）对Th1细胞、Th2细胞、Th17细胞和调节性T细胞（Tregs）的影响以及对胶原诱导性关节炎（CIA）大鼠的干预作用。方法 将用于建立CIA大鼠模型的SD雌性大鼠分为四组，包括CIA模型组、牛Ⅱ型胶原-诱饵-树突状细胞（Col2-诱饵DC）治疗组、空白对照组和Col2-诱饵DC对照组。首次免疫后第20天，经尾静脉注射tolDCs，第7周处死大鼠。采用流式细胞术检测大鼠脾脏中Th1细胞、Th2细胞、Th17细胞和Tregs的比例。通过HE染色评估踝关节病理形态学变化，并对关节炎指数（AI）进行评分。结果 与CIA模型组相比，Col2-诱饵DC组AI较低，踝关节病理形态学变化较轻。脾脏CD4 T细胞中Th1细胞和Th17细胞的百分比降低，而Th2细胞和Tregs的百分比升高。结论 tolDCs治疗可通过降低CD4 T细胞中Th1和Th17细胞的比例减轻CIA大鼠的炎症和关节病变。
