Université Paul Sabatier, CIRIMAT Institut Carnot Chimie Balard CIRIMAT, Faculté de Pharmacie, Toulouse, France; Université Paul Sabatier, I2MC, Toulouse, France.
ITHPP Alcen, Thégra, France.
Carbohydr Polym. 2021 Jun 1;261:117578. doi: 10.1016/j.carbpol.2020.117578. Epub 2021 Feb 22.
Polysaccharidic scaffolds hold great hope in regenerative medicine, however their sterilization still remains challenging since conventional methods are deleterious. Recently, electron beams (EB) have raised interest as emerging sterilization techniques. In this context, the aim of this work was to study the impact of EB irradiations on polysaccharidic macroporous scaffolds. The effects of continuous and pulsed low energy EB were examined on polysaccharidic or on polyelectrolyte complexes (PEC) scaffolds by SEC-MALLS, FTIR and EPR. Then the scaffolds' physicochemical properties: swelling, architecture and compressive modulus were investigated. Finally, sterility and in vitro biocompatibility of irradiated scaffolds were evaluated to validate the effectiveness of our approach. Continuous beam irradiations appear less deleterious on alginate and chitosan chains, but the use of a pulsed beam limits the time of irradiation and better preserve the architecture of PEC scaffolds. This work paves the way for low energy EB tailor-made sterilization of sensitive porous scaffolds.
多糖支架在再生医学中具有很大的应用前景，但由于传统的灭菌方法存在有害影响，其灭菌仍然具有挑战性。最近，电子束（EB）作为新兴的灭菌技术引起了人们的兴趣。在这种情况下，本工作旨在研究 EB 辐照对多糖大孔支架的影响。通过 SEC-MALLS、FTIR 和 EPR 研究了连续和脉冲低能 EB 辐照对多糖或聚电解质复合物（PEC）支架的影响。然后研究了支架的物理化学性质：溶胀、结构和压缩模量。最后，评估了辐照支架的无菌性和体外生物相容性，以验证我们方法的有效性。连续束辐照对海藻酸盐和壳聚糖链的损伤较小，但使用脉冲束限制了辐照时间，并更好地保留了 PEC 支架的结构。这项工作为低能 EB 对敏感多孔支架的定制灭菌铺平了道路。
