Zhu Yulin, Lv Chao, Liu Jingying, Shang Shujun, Jing Wei
Department of Anesthesiology, Yantaishan Hospital, Yantai, Shandong 264000, P.R. China.
Department of Anesthesiology, People's Hospital of Laixi, Laixi, Shandong 266600, P.R. China.
Exp Ther Med. 2021 May;21(5):469. doi: 10.3892/etm.2021.9900. Epub 2021 Mar 8.
The aim of the present study was to investigate the effect of exposure to sevoflurane general anesthesia during early pregnancy on interferon-inducible protein AIM2 (AIM2) expression in the hippocampus and parietal cortex of the offspring Sprague-Dawley (SD) rats. A total of 18 SD rats at a gestational age of 5-7 days were randomly divided into three groups: i) A control group (control); ii) 2-h sevoflurane general anesthesia, group 1 (S1); and iii) 4-h sevoflurane general anesthesia, group 2 (S2). The six offspring rats in each group were maintained for 30 days and assessed by Morris water maze testing. Brain specimens were collected from offspring rats 30 days after birth. Changes in the structural morphology of neurons in the hippocampus and parietal cortex were observed using hematoxylin and eosin staining. Nissl bodies in the hippocampus and parietal cortex were observed by Nissl staining. The expression of glial fibrillary acidic protein (GFAP), AIM2, CD45 and IL-1β was detected by immunohistochemistry and the protein levels of CD45, IL-1β, pro-caspase-1 and caspase-1 p10 were detected by western blotting. Compared with the control group, offspring rats in the S1 and S2 groups exhibited poor long-term learning and memory ability and experienced different degrees of damage to both the hippocampus and parietal cortex. The expression levels of GFAP, AIM2, CD45, IL-1β, caspase-1 and caspase-1 p10 in the offspring of both the S1 and the S2 groups were significantly increased (P<0.05) compared with offspring of the control group. Moreover, compared with the offspring of the S1 group, hippocampal and parietal cortex injury in the offspring of the S2 group was further aggravated, and the expression of GFAP, AIM2, CD45, IL-1β, pro-caspase-1 and cleaved-caspase-1 was significantly increased (P<0.05). In conclusion, sevoflurane general anesthesia in SD rat early pregnancy promoted the expression of AIM2 and the inflammatory response in the hippocampus and parietal cortex of offspring rats.
本研究的目的是探讨孕早期暴露于七氟醚全身麻醉对后代Sprague-Dawley（SD）大鼠海马和顶叶皮质中干扰素诱导蛋白AIM2（AIM2）表达的影响。将18只妊娠5-7天的SD大鼠随机分为三组：i）对照组（control）；ii）2小时七氟醚全身麻醉组，第1组（S1）；iii）4小时七氟醚全身麻醉组，第2组（S2）。每组的6只后代大鼠饲养30天，并通过Morris水迷宫试验进行评估。在出生30天后从后代大鼠收集脑标本。使用苏木精和伊红染色观察海马和顶叶皮质中神经元的结构形态变化。通过尼氏染色观察海马和顶叶皮质中的尼氏体。通过免疫组织化学检测胶质纤维酸性蛋白（GFAP）、AIM2、CD45和IL-1β的表达，并通过蛋白质印迹法检测CD45、IL-1β、前半胱天冬酶-1和半胱天冬酶-1 p10的蛋白质水平。与对照组相比，S1组和S2组的后代大鼠表现出较差的长期学习和记忆能力，并且海马和顶叶皮质均受到不同程度的损伤。与对照组后代相比，S1组和S2组后代中GFAP、AIM2、CD45、IL-1β、半胱天冬酶-１和半胱天冬酶-1 p１０的表达水平均显著升高（P<0.05）。此外，与S1组后代相比，S2组后代的海马和顶叶皮质损伤进一步加重，GFAP、AIM2、CD45、IL-1β、前半胱天冬酶-1和裂解的半胱天冬酶-1的表达显著增加（P<0.05）。总之，SD大鼠孕早期的七氟醚全身麻醉促进了后代大鼠海马和顶叶皮质中AIM2的表达及炎症反应。
