Karamipour Naeime, Fathipour Yaghoub, Mehrabadi Mohammad
Department of Entomology Faculty of Agriculture Tarbiat Modares University Tehran Iran.
Ecol Evol. 2021 Feb 15;11(6):2515-2523. doi: 10.1002/ece3.7188. eCollection 2021 Mar.
The shield bug, (Heteroptera, Pentatomidae), harbors extracellular -like symbiont in the enclosed crypts of the midgut. The symbiotic bacteria are essential for normal longevity and fecundity of this insect. In this study, life table analysis was used to assess the biological importance of the gut symbiont in . Considering vertical transmission of the bacterial symbiont through the egg surface contamination, we used surface sterilization of the eggs to remove the symbiont. The symbiont population was decreased in the newborn nymphs hatched from the surface-sterilized eggs (the aposymbiotic insects), and this reduction imposed strongly negative effects on the insect host. We found significant differences in most life table parameters between the symbiotic insects and the aposymbiotics. The intrinsic rate of increase in the control insects (0.080 ± 0.003 day) was higher than the aposymbiotic insects (0.045 ± 0.007 day). Also, the net reproductive and gross reproductive rates were decreased in the aposymbiotic insects (i.e., 20.770 ± 8.992 and 65.649 ± 27.654 offspring/individual, respectively), compared with the symbiotic insects (i.e., 115.878 ± 21.624 and 165.692 ± 29.058 offspring/individual, respectively). These results clearly show biological importance of the symbiont in .
盾蝽（半翅目，蝽科）在中肠封闭的隐窝中含有细胞外样共生菌。这些共生细菌对于这种昆虫的正常寿命和繁殖力至关重要。在本研究中，通过生命表分析来评估肠道共生菌在盾蝽中的生物学重要性。考虑到细菌共生菌通过卵表面污染进行垂直传播，我们对卵进行表面消毒以去除共生菌。从表面消毒的卵（即aposymbiotic昆虫）孵化出的新生若虫中，共生菌数量减少，这种减少对昆虫宿主产生了强烈的负面影响。我们发现共生昆虫和aposymbiotic昆虫在大多数生命表参数上存在显著差异。对照昆虫的内禀增长率（0.080±0.003天）高于aposymbiotic昆虫（0.045±0.007天）。此外，与共生昆虫（分别为115.878±21.624和165.692±29.058个后代/个体）相比，aposymbiotic昆虫的净生殖率和总生殖率降低（分别为20.770±8.992和65.649±27.654个后代/个体）。这些结果清楚地表明了共生菌在盾蝽中的生物学重要性。
