Schimmack L, Kaden J, Hackensellner H A
Z Exp Chir. 1977;10(5):275-84.
The authors report investigations on inducing lymphocytotoxic antibodies by transplanting allogenic skin grafts in mice after different kinds of preservation. Immunogenic effect was found at the following test conditions: storage of the graft for 4 weeks at -196 degrees C without cryoprotection, storage for 1 day at -38 degrees C, and storage at room temperature after lyophilization immediately after removal. No immunogenity was found after storage for 3 weeks at -38 degrees C, and uncertain immunogenity after storage for 4 weeks in 4 per cent formaldehyde solution. Sometimes the average strength of the microcyte toxin test was lowered around the 7th to 9th day after transplantation. That is interpreted as sign of adsorption of circulating antibodies to the graft. The authors point at the coincidence of these findings and the rejection of primary grafts, and at the potential causal relations between both phenomena.
作者报告了关于在不同保存方式后将同种异体皮肤移植到小鼠体内诱导淋巴细胞毒性抗体的研究。在以下试验条件下发现了免疫原性效应：移植物在-196℃无冷冻保护剂的情况下储存4周、在-38℃储存1天以及取出后立即冻干并在室温下储存。在-38℃储存3周后未发现免疫原性，在4%甲醛溶液中储存4周后免疫原性不确定。有时在移植后第7至9天左右，微细胞毒素试验的平均强度会降低。这被解释为循环抗体吸附到移植物上的迹象。作者指出了这些发现与原发性移植物排斥之间的巧合，以及这两种现象之间潜在的因果关系。
