Opt Express. 2021 Feb 1;29(3):4159-4173. doi: 10.1364/OE.410878.
In this paper, we present a method to distinguish neoplastic tissues from non-neoplastic ones using calibration-free laser-induced breakdown spectroscopy (CF-LIBS). For this propose, plasma emission was collected from neoplastic and non-neoplastic tissues taken from the ovarian cancer mice models. Results were obtained by utilizing the characteristic plasma emission lines of different elements that have been confirmed in the investigated samples. From the temporal evolution of plasma emission, the optimum temporal-observation-windows are identified for LIBS investigation. The concentrations of the detected elements in tissues were measured by a calibration-free approach based on data process of plasma parameters at the local thermodynamic equilibrium. The neoplastic specimens provided more energetic plasma than non-neoplastic ones that resulting in higher peaks intensities, electron density and electron temperature especially in the early windows (between 0.1 µs to 0.8 µs). Results demonstrated higher concentrations of major and trace elements such as Mg, Fe, Ca, Na, and K in the neoplastic tissues. Finally, the results using CF-LIBS method were found to be in good agreement with that of Inductive coupled plasma-optical emission spectroscopy (ICP-OES).
在本文中，我们提出了一种使用无校准激光诱导击穿光谱（CF-LIBS）来区分肿瘤组织和非肿瘤组织的方法。为此，从卵巢癌小鼠模型中采集了肿瘤和非肿瘤组织的等离子体发射。通过利用已在研究样本中确认的不同元素的特征等离子体发射线来获得结果。从等离子体发射的时间演化中，确定了用于 LIBS 研究的最佳时间观测窗口。通过基于局部热力学平衡下等离子体参数数据处理的无校准方法测量组织中检测到的元素的浓度。肿瘤标本比非肿瘤标本提供了更具活力的等离子体，从而导致更高的峰值强度、电子密度和电子温度，特别是在早期窗口（0.1µs 到 0.8µs 之间）。结果表明，肿瘤组织中存在更高浓度的主要和微量元素，如 Mg、Fe、Ca、Na 和 K。最后，发现 CF-LIBS 方法的结果与电感耦合等离子体-光学发射光谱（ICP-OES）的结果非常吻合。
