Heuke Sandro, Rigneault Hervé, Sentenac Anne
Opt Express. 2021 Feb 1;29(3):4230-4239. doi: 10.1364/OE.416290.
Fourier ptychography tomography (FPT) is a novel computational technique for coherent imaging in which the sample is numerically reconstructed from images acquired under various illumination directions. FPT is able to provide three-dimensional (3D) reconstructions of the complex sample permittivity with an increased resolution compared to standard microscopy. In this work, FPT is applied to coherent anti-Stokes Raman scattering (CARS) imaging. We show on synthetic data that complex third-order susceptibilities can be reconstructed in 3D from a limited number of widefield CARS images. In addition, we observe that the non-linear interaction increases significantly the potential of CARS-FPT compared to linear FPT in terms of resolution. In particular, with a careful choice of the pump and Stokes beam directions, CARS-FPT is able to provide optical sectioning even in transmission configuration.
傅里叶叠层成像术（FPT）是一种用于相干成像的新型计算技术，在该技术中，通过在不同照明方向下采集的图像对样本进行数值重建。与标准显微镜相比，FPT能够以更高的分辨率提供复杂样本介电常数的三维（3D）重建。在这项工作中，FPT被应用于相干反斯托克斯拉曼散射（CARS）成像。我们在合成数据上表明，可以从有限数量的宽场CARS图像中三维重建复三阶磁化率。此外，我们观察到，与线性FPT相比，非线性相互作用显著提高了CARS-FPT在分辨率方面的潜力。特别是，通过精心选择泵浦光和斯托克斯光束的方向，CARS-FPT即使在透射配置下也能够提供光学切片。
