Hajek Kim M, Littleton Brad, Turk Douglas, McIntyre Timothy J, Rubinsztein-Dunlop Halina
Centre for Biophotonics and Laser Science, School of Mathematics and Physics, The University of Queensland, Brisbane, Queensland 4072, Australia.
Opt Express. 2010 Aug 30;18(18):19263-72. doi: 10.1364/OE.18.019263.
We propose a scheme for achieving widefield coherent anti-Stokes Raman scattering (CARS) microscopy images with sub-diffraction-limited resolution. This approach adds structured illumination to the widefield CARS configuration [Applied Physics Letters 84, 816 (2004)]. By capturing a number of images at different phases of the standing wave pattern, an image with up to three times the resolution of the original can be constructed. We develop a theoretical treatment of this system and perform numerical simulations for a typical CARS system, which indicate that resolutions around 120 nm are obtainable with the present scheme. As an imaging system, this method combines the advantages of sub-diffraction-limited resolution, endogenous contrast generation, and a wide field of view.
我们提出了一种用于实现具有亚衍射极限分辨率的宽场相干反斯托克斯拉曼散射（CARS）显微镜图像的方案。这种方法将结构化照明添加到宽场CARS配置中[《应用物理快报》84, 816 (2004)]。通过在驻波图案的不同相位捕获多个图像，可以构建出分辨率高达原始图像三倍的图像。我们对该系统进行了理论处理，并对典型的CARS系统进行了数值模拟，结果表明采用本方案可获得约120 nm的分辨率。作为一种成像系统，该方法兼具亚衍射极限分辨率、内源性对比度生成和宽视场的优点。
