Department of Biology, Campus Santiago, University of Aveiro, 3810-193 Aveiro, Portugal; CESAM, Campus Santiago, University of Aveiro, 3810-193 Aveiro, Portugal.
Department of Biology, Campus Santiago, University of Aveiro, 3810-193 Aveiro, Portugal; CESAM, Campus Santiago, University of Aveiro, 3810-193 Aveiro, Portugal.
Gene. 2021 Jun 15;785:145603. doi: 10.1016/j.gene.2021.145603. Epub 2021 Mar 23.
Vegetables may become contaminated with antibiotic-resistant bacteria from farm-to-fork. Here we report draft genome sequences of two multidrug-resistant Escherichia coli isolated from lettuce. Whole genomes of strains Y15 V.22 and Y15 V.54 were sequenced. Available tools were used to inspect for virulence factors (VF), metals tolerance, resistome and mobilome features. The predicted genome sizes were 5,4 Mb and 6,2 Mb for Y15 V.22 and Y15 V.54, respectively, both with 50.7% GC content, ST48 and serotype O8:H9. Resistome analysis showed genes encoding resistance to β-lactams, sulphonamides, trimethoprim, tetracyclines and macrolides. Cobalt, cadmium, zinc and copper tolerance determinants were identified in both. VF detected included genetic determinants related to toxin production, adherence and invasion. SNPs and VF content analysis showed a close relatedness to ETEC. Putative genomic islands, prophage and CRISPR sequences were predicted. The genome sequences here reported will aid in understanding antibiotic resistance transfer between vegetables consumed raw and humans.
蔬菜可能会在从农场到餐桌的过程中被抗生素耐药细菌污染。在这里，我们报告了从生菜中分离出的两株多药耐药大肠杆菌 Y15 V.22 和 Y15 V.54 的基因组序列草案。对菌株 Y15 V.22 和 Y15 V.54 的全基因组进行了测序。利用现有工具检测毒力因子 (VF)、耐金属性、耐药组和可移动组特征。Y15 V.22 和 Y15 V.54 的预测基因组大小分别为 5.4 Mb 和 6.2 Mb，GC 含量均为 50.7%，血清型分别为 ST48 和 O8:H9。耐药组分析显示，编码对β-内酰胺类、磺胺类、甲氧苄啶、四环素类和大环内酯类药物耐药的基因。两者都存在钴、镉、锌和铜耐定性决定因素。检测到的 VF 包括与毒素产生、粘附和侵袭相关的遗传决定因素。SNP 和 VF 含量分析表明与肠致病性大肠杆菌 (ETEC) 密切相关。预测了潜在的基因组岛、噬菌体和 CRISPR 序列。本报告的基因组序列将有助于了解生食蔬菜和人类之间抗生素耐药性的转移。
