Shiojiri N, Katayama H
Biological Institute, Faculty of Science, Shizuoka University, Japan.
Anat Embryol (Berl). 1988;178(1):15-20. doi: 10.1007/BF00305009.
The distribution of binding sites for Dolichos biflorus agglutinin (DBA) was studied by histochemical staining in the embryonic mouse liver. The liver primordium consists of cranial and caudal diverticula. DBA bound to the pseudostratified endodermal cells of the caudal hepatic diverticulum, and also to some endodermal cells of the cranial one. Most extrahepatic bile duct cells and all epithelial cells of the gall bladder were consistently DBA-positive. In intrahepatic bile ducts and their precursors, the DBA binding sites showed a patchy distribution. Most hepatocytes were DBA-negative, though some young hepatocytes were DBA-positive. The results suggest that DBA binding sites are useful markers of epithelial cells of the gall bladder and the bile ducts, especially the extrahepatic bile duct. Differentiation of bile duct cells was also discussed with special reference to the developmental change of the distribution of the DBA binding sites.
通过组织化学染色研究了双花扁豆凝集素（DBA）在胚胎小鼠肝脏中的结合位点分布。肝原基由头侧和尾侧憩室组成。DBA与尾侧肝憩室的假复层内胚层细胞结合，也与头侧肝憩室的一些内胚层细胞结合。大多数肝外胆管细胞和胆囊的所有上皮细胞始终呈DBA阳性。在肝内胆管及其前体中，DBA结合位点呈斑片状分布。大多数肝细胞为DBA阴性，不过一些年轻肝细胞为DBA阳性。结果表明，DBA结合位点是胆囊和胆管上皮细胞，尤其是肝外胆管上皮细胞的有用标记物。还特别参考DBA结合位点分布的发育变化讨论了胆管细胞的分化。
