Gupta Sonam, Gupta Payal, Pruthi Vikas
Department of Biotechnology, National Institute of Technology Raipur, Raipur, 492001, Chhattisgarh, India.
Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, 247667, Uttarakhand, India.
Curr Microbiol. 2021 May;78(5):1813-1822. doi: 10.1007/s00284-021-02461-5. Epub 2021 Mar 27.
In the present investigation, we have evaluated the antibiofilm potential of Bacillus licheniformis SV1 derived glycolipid against C. glabrata biofilm. Impact of isolated glycolipid on the viability of C. glabrata and on inhibiting as well as eradicating ability of its biofilm were studied. Further, morphological alterations, reactive oxygen species generation (ROS) production and transcriptional expression of selected genes (RT-PCR) of C. glabrata in response with isolated glycolipid were studied. The isolated glycolipid (1.0 mg ml) inhibited and eradicated C. glabrata biofilm approximately 80% and 60%, respectively. FE-SEM images revealed glycolipid exposure results in architectural alteration and eradication of C. glabrata biofilm and ROS generation. Transcriptional studies of selected genes showed that the expression of AUS1, FKS1 and KRE1 were down-regulated, while that of ergosterol biosynthesis pathway and multidrug transporter increased, in the presence of glycolipid.
在本研究中，我们评估了地衣芽孢杆菌SV1产生的糖脂对光滑念珠菌生物膜的抗生物膜潜力。研究了分离出的糖脂对光滑念珠菌活力及其生物膜抑制和根除能力的影响。此外，还研究了光滑念珠菌在接触分离出的糖脂后的形态变化、活性氧（ROS）生成以及选定基因的转录表达（RT-PCR）。分离出的糖脂（1.0 mg/ml）分别抑制和根除了约80%和60%的光滑念珠菌生物膜。场发射扫描电子显微镜（FE-SEM）图像显示，糖脂处理导致光滑念珠菌生物膜结构改变、被根除以及ROS生成。选定基因的转录研究表明，在糖脂存在的情况下，AUS1、FKS1和KRE1的表达下调，而麦角固醇生物合成途径和多药转运蛋白的表达增加。
