Department of Histology and Embryology, Faculty of Veterinary Medicine, Erciyes University, Kayseri, Turkey.
Anat Histol Embryol. 2021 May;50(3):645-657. doi: 10.1111/ahe.12672. Epub 2021 Mar 27.
The mammalian oviduct provides a favourable environment for several reproductive processes, including ovum transport, sperm capacitation, fertilization and pre-implantation embryonic development. This environment is regulated by cyclic ovarian steroids, that is oestrogen, and growth factors. Fibroblast growth factors (FGFs) regulate the differentiation and growth of various cell types in the female genital tract. This study aimed to determine the localization of FGF1, FGF2, FGF receptor 1 (FGFR1) and 2 (FGFR2) in the rat oviduct, by immunohistochemistry, on day 5 of pregnancy and post-partum days 1, 3 and 5, and to demonstrate the possible functions of these proteins during early pregnancy and the post-partum period. On all examination days, cytoplasmic and nuclear FGF1 immunoreactivity was detected in the epithelium lining the infundibulum, ampulla and isthmus of the oviduct. Immunoreactivity was much stronger in the basal bodies of the cilia on the epithelium lining the infundibulum and ampulla. FGF1 immunoreactivity was also detected in stromal cells, myocytes and endothelial cells. Cytoplasmic FGF2 immunoreactivity was observed in the tunica muscularis, vascular myocytes and endothelial cells. While strong cytoplasmic FGF2 immunoreactions were observed in the stromal cells of the lamina propria, the luminal epithelium, some stromal cells and smooth muscle cells displayed a rather weak FGFR1 and FGFR2 immunoreactivity. Immunoreaction intensity did not differ between the periods examined. This study shows that FGF1, FGF2, FGFR1 and FGFR2 are produced by rat oviduct cells during pregnancy and the post-partum period, and reproductive physiology is regulated not only by hormonal mechanisms, but also by growth factors.
哺乳动物输卵管为几个生殖过程提供了有利的环境，包括卵子运输、精子获能、受精和着床前胚胎发育。这种环境受周期性卵巢类固醇调节，即雌激素和生长因子。成纤维细胞生长因子（FGFs）调节女性生殖道各种细胞类型的分化和生长。本研究旨在通过免疫组织化学方法，在妊娠第 5 天和产后第 1、3、5 天，确定 FGF1、FGF2、FGFR1 和 FGFR2 在大鼠输卵管中的定位，并证明这些蛋白在早孕和产后期间的可能功能。在所有检查日，在输卵管漏斗、壶腹和峡部的上皮衬里中检测到细胞质和核 FGF1 免疫反应性。在漏斗和壶腹上皮衬里的纤毛基底小体中，免疫反应性更强。FGF1 免疫反应性也在基质细胞、肌细胞和内皮细胞中检测到。在肌层中观察到细胞质 FGF2 免疫反应性，血管肌细胞和内皮细胞。而在固有层的基质细胞中观察到强烈的细胞质 FGF2 免疫反应性，一些基质细胞和平滑肌细胞显示出较弱的 FGFR1 和 FGFR2 免疫反应性。在检查的各个时期，免疫反应强度没有差异。本研究表明，FGF1、FGF2、FGFR1 和 FGFR2 在妊娠和产后期间由大鼠输卵管细胞产生，生殖生理学不仅受激素机制调节，还受生长因子调节。
