Department of Pharmaceutical Sciences, North South University, Dhaka, Bangladesh; Department of Pharmacy, International Islamic University Chittagong, Chittagong 4318, Bangladesh.
Department of Pharmaceutical Sciences, North South University, Dhaka, Bangladesh.
Biomed Pharmacother. 2021 Jun;138:111530. doi: 10.1016/j.biopha.2021.111530. Epub 2021 Mar 24.
Aphanamixis polystachya (Wall.) R.Parker, locally known as Pithraj, is a medicinal herb having enormous traditional applications. However, the scientific rationale underlying the ethnomedicinal claims was not well-founded. The current investigation aimed to explore the mechanistic insights of protective effects of ethanol extract of A. polystachya leaf (PT), given orally, on the chemical-intoxicated hepatic inflammation and fibrosis in Long-Evans female overiectomized rats. The GC-MS and HPLC-DAD analysis of PT revealed the presence of several bioactive metabolites, including polyphenolic compounds. Catechin hydrate, caffeic acid, syringic acid, epicatechin and p-coumaric acid have been identified and quantified in the ethanol extract of PT leaf. Intoxication with CCl developed the oxidative stress, fibrosis and inflammation in liver of rats. Moreover, thiobarbituric acid reactive substances (TBARS), nitric oxide (NO), advanced protein oxidation product (APOP) level were found increased; whereas superoxide dismutase (SOD) and catalase activities in the plasma and liver were decreased in CCl administered rats. Treatment with PT prominently mitigated the oxidative stress (TBARS, NO, APOP), and inflammatory (MPO) markers and improved the endogenous antioxidant enzymes (catalase and SOD) activities in CCl-intoxicated rats. Additionally, histological assessment confirmed the clear manifestation of inflammation and fibrosis in the liver of CCl-intoxicated rats, which was prevented by PT and silymarin treatment. In conclusion, PT treatment may protect the liver in CCl-administered rats, probably by mitigating oxidative stress, inflammation and fibrosis, and also augmenting the function of the antioxidant enzymes.
多穗柯（Wall.）R.Parker，俗称皮茶，是一种具有巨大传统应用价值的药用植物。然而，其民族医学主张的科学依据并不充分。本研究旨在探索多穗柯叶乙醇提取物（PT）经口给予对长耳雌性去卵巢大鼠化学性肝炎症和纤维化的保护作用的机制见解。PT 的 GC-MS 和 HPLC-DAD 分析表明，存在几种生物活性代谢物，包括多酚类化合物。儿茶素水合物、咖啡酸、丁香酸、表儿茶素和对香豆酸已在 PT 叶乙醇提取物中被鉴定和定量。CCL 中毒导致大鼠肝脏氧化应激、纤维化和炎症。此外，丙二醛（TBARS）、一氧化氮（NO）、高级蛋白质氧化产物（APOP）水平升高；而 CCl 给药大鼠血浆和肝脏中超氧化物歧化酶（SOD）和过氧化氢酶活性降低。PT 治疗明显减轻了 CCl 中毒大鼠的氧化应激（TBARS、NO、APOP）和炎症（MPO）标志物，并改善了内源性抗氧化酶（过氧化氢酶和 SOD）的活性。此外，组织学评估证实了 CCl 中毒大鼠肝脏炎症和纤维化的明显表现，PT 和水飞蓟素治疗可预防这种表现。总之，PT 治疗可能通过减轻氧化应激、炎症和纤维化，以及增强抗氧化酶的功能来保护 CCl 给药大鼠的肝脏。
