Department of Sustainable Crop Production, Università Cattolica del Sacro Cuore, Via Emilia Parmense 84, 29122 Piacenza, Italy.
Department for Sustainable Food Process, Università Cattolica del Sacro Cuore, Via Emilia Parmense 84, 29122 Piacenza, Italy.
Food Res Int. 2021 Apr;142:110212. doi: 10.1016/j.foodres.2021.110212. Epub 2021 Feb 14.
In this work, considering the rising interest towards the exploitation of hemp seed oil in human nutrition, 45 hemp seeds from mono-variety fields were analyzed for their yield, oil content, in vitro antioxidant activity, followed by a comprehensive assessment of phenolic and sterolic composition. The results demonstrated that seed dimension is inversely correlated to total oil content, thus being a potential reference for quality assessment of seeds and for further improvement of hemp varieties. The UHPLC-QTOF metabolomic analysis revealed a large abundance of phytosterols, lower-molecular-weight phenolic acids, and lignanamides. Differences across varieties could be described, with Diana hemp seed oil having the highest cumulative abundance of phytochemicals, recording 6.04 mg/g. Overall, the in vitro antioxidant activity results indicated that hemp seed oil antioxidants have a low potential for preventing oil rancidity, with phenolic acids being the most active radical scavengers. Besides, in the group of Futura 75 samples cultivated across Italy, the type of harvesting affected the acidity value significantly as a consequence of mechanical harvest and post-harvest handling. Finally, multivariate statistics following untargeted metabolomic analysis showed that variety, geographical origin, and harvest-type were able to affect the phytochemical profiles with different incidences, with some phytochemicals proposed for the first time as potential discriminant markers.
在这项工作中，考虑到人们对开发大麻籽油在人类营养中的兴趣日益浓厚，对来自单一品种田地的 45 颗大麻种子进行了分析，以了解其产量、油含量、体外抗氧化活性，随后对酚类和甾醇组成进行了综合评估。结果表明，种子的尺寸与总油含量成反比，因此可作为种子质量评估和进一步改良大麻品种的潜在参考。UHPLC-QTOF 代谢组学分析显示，植物甾醇、低分子量酚酸和木质素酰胺的含量丰富。不同品种之间存在差异，Diana 大麻籽油中酚类化合物的总含量最高，达到 6.04mg/g。总的来说，体外抗氧化活性结果表明，大麻籽油中的抗氧化剂预防油变质的潜力较低，其中酚酸是最活跃的自由基清除剂。此外，在意大利种植的 Futura 75 样本组中，由于机械收获和收获后处理，收获方式显著影响了酸度值。最后，非靶向代谢组学分析后的多元统计表明，品种、地理起源和收获方式能够以不同的频率影响植物化学特征，一些植物化学物质首次被提议作为潜在的鉴别标志物。
