Bioprocess Laboratory, Department of Microbial Biotechnology, School of Biotechnology and Genetic Engineering, Bharathiar University, Coimbatore, India.
Division of Environment, United Carbon Solutions Pvt Ltd, Tiruppur, Tamil Nadu, India.
Chemosphere. 2021 Aug;277:130311. doi: 10.1016/j.chemosphere.2021.130311. Epub 2021 Mar 17.
In this study, we aimed to assess the possible reusability of native and surface-modified waste biomass of a novel ascomycetes fungi Trichoderma asperellum BPL MBT1 for the adsorption of triphenylmethane dyes. Spent biomass obtained from fermentation medium has been applied in the uptake of model cationic dyes viz., crystal violet and malachite green. Optimization of experimental parameters by batch mode studies revealed that dye adsorption is influenced by medium pH time, initial concentration of dyes, and adsorbent dosage. It was observed that pH 10 was optimum for cationic dye adsorption. Further, the adsorption process obeyed the bi-model (Langmuir-Freundlich model) isotherm and adhered to pseudo-second-order kinetics. The involvement of ion exchange as the dominant mechanism of dye adsorption was indicated by the mean free energy obtained from Dubinin-Radushkevich isotherm. Cellular morphology and the involved functional groups were studied by scanning electron microscopy and Fourier transform infrared spectroscopy that revealed the presence of carbon and oxygen containing groups on the surface. Maximum desorption efficiency was achieved using a 0.1 M solution of HCl and the stability of the biosorbent was confirmed through reusability analysis. Our results confirm the applicability of both native and surface-modified T. asperellum BPL MBT1 biomass as a potential biosorbent for the sustainable wastewater treatment and safe dye disposal.
在这项研究中，我们旨在评估新型曲霉菌 Aspergillus asperellum BPL MBT1 的原生和表面改性废生物质对三苯甲烷染料的吸附的可能可重复使用性。从发酵培养基中获得的废生物质已应用于模型阳离子染料，即结晶紫和孔雀石绿的吸收。通过分批模式研究优化实验参数表明，染料吸附受介质 pH 值、时间、染料初始浓度和吸附剂用量的影响。结果表明，pH 值为 10 时阳离子染料的吸附效果最佳。此外，吸附过程符合双模型（朗缪尔-弗伦德利希模型）等温线并符合拟二级动力学。从杜宾宁-拉什凯维奇等温线获得的平均自由能表明，离子交换是染料吸附的主要机制。通过扫描电子显微镜和傅里叶变换红外光谱研究了细胞形态和涉及的官能团，结果表明表面存在碳和含氧基团。使用 0.1 M 的 HCl 溶液可实现最大的解吸效率，并通过可重复使用性分析证实了生物吸附剂的稳定性。我们的结果证实了原生和表面改性 T. asperellum BPL MBT1 生物质作为可持续废水处理和安全染料处理的潜在生物吸附剂的适用性。
