TERI-Deakin Nano Biotechnology Centre, The Energy and Resources Institute, Darbari Seth Block, IHC Complex, Lodhi Road, New Delhi 110003, India.
TERI-Deakin Nano Biotechnology Centre, The Energy and Resources Institute, Darbari Seth Block, IHC Complex, Lodhi Road, New Delhi 110003, India.
J Mycol Med. 2021 Jun;31(2):101133. doi: 10.1016/j.mycmed.2021.101133. Epub 2021 Mar 18.
A total of 70 feathers samples of Emu (Dromaius novaehollandiae) were collected from 7 Emu farms situated at two districts (Raigad and Thane) of Maharashtra (India) and screened for resident keratinophilic fungi. Among them, 44 isolates were recovered and identified by evaluating characteristic macro- and micro-morphological features. Further gene products corresponding to the ITS1-5.8S-ITS2 rDNA region from all isolates were amplified and sequenced. Homology search was performed using BLAST program against non-redundant nucleotide database, and significantly matched DNA sequences deposited to the NCBI Gene Bank for reference purposes. Eight identified fungal species belongs to 7 different genera named as Aphanoascus terreus Ac_MW577456 (21.43%), Microsporum gypseum Ac_MW580920 (14.29%), Ctenomyces serratus Ac_MW577459 (10.0%), Uncinocarpus orissi Ac_MW577461 (5.17%), Aphanoascus verrucosus Ac_MW577458 (4.29%), Gymnascella dankaliensis Ac_MW577460 (2.86%), Gymnoascoideus petalosporus Ac_MW577462 (2.86%) and Arthroderma tuberculatum Ac_MW577457 (1.43%).
从印度马哈拉施特拉邦（Maharashtra）的两个地区（Raigad 和 Thane）的 7 个鸸鹋农场收集了总共 70 个鸸鹋（Dromaius novaehollandiae）羽毛样本，并对其常驻角蛋白酶真菌进行了筛查。其中，从 44 个分离物中恢复并通过评估特征性宏观和微观形态特征进行了鉴定。进一步从所有分离物中扩增和测序了对应于 ITS1-5.8S-ITS2 rDNA 区域的基因产物。使用 BLAST 程序对同源性搜索进行了非冗余核苷酸数据库，并且为了参考目的，将显著匹配的 DNA 序列提交到 NCBI 基因银行中进行了存储。鉴定出的 8 种真菌物种属于 7 个不同属，分别为 Aphanoascus terreus Ac_MW577456（21.43％）、Microsporum gypseum Ac_MW580920（14.29％）、Ctenomyces serratus Ac_MW577459（10.0％）、Uncinocarpus orissi Ac_MW577461（5.17％）、Aphanoascus verrucosus Ac_MW577458（4.29％）、Gymnascella dankaliensis Ac_MW577460（2.86％）、Gymnoascoideus petalosporus Ac_MW577462（2.86％）和 Arthroderma tuberculatum Ac_MW577457（1.43％）。
