State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China; Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insect Pests, Zhejiang University, Hangzhou 310058, China; Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province, Zhejiang University, Hangzhou 310058, China.
State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China.
Curr Opin Virol. 2021 Jun;48:1-9. doi: 10.1016/j.coviro.2021.03.003. Epub 2021 Mar 25.
Our understanding of the biology and pathogenesis of plant negative-stranded RNA viruses (NSVs) has lagged behind those made with positive-stranded RNA and DNA virus counterparts. This tardiness is mainly due to the lack of reverse genetics tools for NSV genome engineering for many years. The eventual establishment and application of recombinant systems with diverse plant NSVs has provided renewed momentum for investigations of these important viral pathogens. In this review, we summarize the recent advances in plant NSV reverse genetics systems, highlighting the general principles and the uniqueness of each system and emphasizing important considerations for strategy designing. We also provide a brief overview of the insights about NSV morphogenesis, movement, and virus-host interactions gained from reverse genetics-enabled studies.
我们对植物负义单链 RNA 病毒（NSV）的生物学和发病机制的理解落后于正义单链 RNA 和 DNA 病毒的对应物。这种滞后主要是由于多年来缺乏用于 NSV 基因组工程的反向遗传学工具。最终建立和应用具有多种植物 NSV 的重组系统为这些重要的病毒病原体的研究提供了新的动力。在这篇综述中，我们总结了植物 NSV 反向遗传学系统的最新进展，重点介绍了每个系统的一般原则和独特性，并强调了策略设计的重要考虑因素。我们还简要概述了通过反向遗传学研究获得的关于 NSV 形态发生、运动和病毒-宿主相互作用的见解。
