Department of Natural Products, National Institute of Pharmaceutical Education and Research, Kolkata, 700054, India.
Department of Chemistry, University of Calcutta, Kolkata, 700009, India.
Mol Biotechnol. 2021 Jun;63(6):459-476. doi: 10.1007/s12033-021-00310-1. Epub 2021 Mar 27.
Clustered regularly interspaced short palindromic repeats (CRISPR) and their associated Cas protein technology area is rapidly growing technique for genome editing and modulation of transcription of several microbes. Successful engineering in microbes requires an emphasis on the aspect of efficiency and targeted aiming, which can be employed using CRISPR/Cas system. Hence, this type of system is used to modify the genome of several microbes such as yeast and bacteria. In recent years, CRISPR/Cas systems have been chosen for metabolic engineering in microbes due to their specificity, orthogonality, and efficacy. Therefore, we need to review the scheme which was acquired for the execution of the CRISPR/Cas system for the modification and metabolic engineering in yeast and bacteria. In this review, we highlighted the application of the CRISPR/Cas system which has been used for the production of small molecules in the microbial system that is chemically and biologically important.
成簇规律间隔短回文重复序列 (CRISPR) 及其相关的 Cas 蛋白技术领域是用于基因组编辑和几种微生物转录调控的快速发展技术。成功的微生物工程需要强调效率和靶向性方面，可以使用 CRISPR/Cas 系统来实现。因此，这种系统被用于修饰酵母和细菌等几种微生物的基因组。近年来，由于 CRISPR/Cas 系统的特异性、正交性和有效性，该系统已被用于微生物的代谢工程。因此，我们需要回顾一下用于修饰酵母和细菌的 CRISPR/Cas 系统的执行方案。在本综述中，我们重点介绍了 CRISPR/Cas 系统在微生物系统中用于生产具有重要化学和生物学意义的小分子方面的应用。
