Department of Biomedical Laboratory Science and Management and Clinical Nutrition and Dietetics Division, (UGC Innovative Department), Vidyasagar University, Midnapore, West Bengal, 721102, India.
Food Safety, ICMR National Institute of Nutrition, Hyderabad, India.
Environ Sci Pollut Res Int. 2021 Aug;28(30):41095-41108. doi: 10.1007/s11356-021-13457-w. Epub 2021 Mar 28.
The non-invasive treatment strategy is indispensable to overcome the side effects of conventional treatment with chelating agents against arsenic. Presence of catechins and flavonoids in Camellia sinensis have potential antioxidant properties and other beneficial effects. The aim of the study was to explore the curative potential role of Camellia sinensis against uterine damages produced by sodium arsenite in mature albino rats. A dose of 10 mg of Camellia sinensis ethyl acetate (CS-EA) fraction/100 gm body weight was provided to the sodium arsenite-treated rats (10 mg/Kg body weight). LC-MS analysis was used for the detection of active component in CS-EA fraction. Enzymatic antioxidants analysis carried out by reproducible native gel technique. Hormones and some pro and anti-inflammatory markers were detected by ELISA, PCR, and western blot techniques respectively. Immunostaining was performed for the detection of estradiol receptor alpha. LC-MS analysis of CS-EA fraction ensured the presence of active tea polyphenol and tea catechin of which highest peak of epigallocatechin-3 gallate (EGCG) was obtained in this study. Significant elevations of lipid peroxidation end products followed by the diminution of antioxidant enzymes activities were noted in arsenicated rats which were capably retrieved by the treatment of CS-EA fraction. Post-treatment with CS-EA fraction meaningfully improved gonadotrophins and estradiol signalling in association with a highly expressing estradiol receptor-α (ERα) in the ovary and uterus followed by the maintenance of normal utero-ovarian histoarchitecture in arsenic fed rats. CS-EA fractioned treated group overturned the sodium arsenite driven higher expression of pro-inflammatory cytokines and proapoptotic markers along with a low level of anti apoptotic Bcl-2 expression and comparatively lower NF-κB signalling in the uterus via regulating IKK β kinase mostly by EGCG of CS-EA fraction. However, ethyl acetate fraction of Camellia sinensis played a critical role in minimizing arsenic-mediated uterine hypo-function.
针对砷的螯合物常规治疗的副作用，非侵入性治疗策略是不可或缺的。儿茶素和类黄酮存在于茶树中，具有潜在的抗氧化特性和其他有益作用。本研究旨在探索茶树乙酸乙酯（CS-EA）部分对亚砷酸钠引起的成熟白化大鼠子宫损伤的治疗潜力。向亚砷酸钠处理的大鼠（10mg/Kg 体重）提供 10mg 的茶树乙酸乙酯（CS-EA）部分/100gm 体重的剂量。LC-MS 分析用于检测 CS-EA 部分中的活性成分。通过可重复的天然凝胶技术进行酶促抗氧化剂分析。通过 ELISA、PCR 和 western blot 技术分别检测激素和一些促炎和抗炎标志物。免疫染色用于检测雌激素受体α。CS-EA 部分的 LC-MS 分析确保了存在活性茶多酚和儿茶素，其中表没食子儿茶素-3-没食子酸酯（EGCG）的峰最高。砷化大鼠的脂质过氧化终产物显著升高，抗氧化酶活性降低，经 CS-EA 部分处理后得以恢复。CS-EA 部分的治疗后显著改善了促性腺激素和雌激素信号转导，与卵巢和子宫中雌激素受体-α（ERα）的高表达相关，并随后维持了砷喂养大鼠的正常子宫-卵巢组织学结构。CS-EA 部分处理组通过调节 IKKβ激酶，主要是通过 CS-EA 部分的 EGCG，逆转了促炎细胞因子和促凋亡标志物的高表达以及抗凋亡 Bcl-2 表达水平低和 NF-κB 信号转导水平低，这些在子宫中是由亚砷酸钠驱动的。然而，茶树的乙酸乙酯部分在最小化砷介导的子宫功能低下方面发挥了关键作用。
