一株新建立的犬自然杀伤细胞系及其细胞毒性。
A newly established canine NK-type cell line and its cytotoxic properties.
机构信息
Laboratory of Tumor Molecular Immunobiology, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Wroclaw, Poland.
Department of Biochemistry, Pharmacology and Toxicology, Wroclaw University of Environmental and Life Sciences, Wroclaw, Poland.
出版信息
Vet Comp Oncol. 2021 Sep;19(3):567-577. doi: 10.1111/vco.12695. Epub 2021 Apr 26.
We established a canine natural killer (NK)-type cell line called CNK-89 derived from a dog with NK cell neoplasia. Immunophenotyping analysis showed positive staining for CD5, CD8, CD45, CD56, CD79a and NKp46, while negative for CD3, CD4, CD14, CD20, CD21, CD34, Thy1, IgG, IgM and MHCII. Polymerase chain reaction analysis revealed the presence of CD56, NKG2D, NKp30, NKp44, NKp46 and perforin, but the absence of CD16, Ly49 and granzyme B mRNA. Treating CNK-89 cells with IL-2 did not change the expression of activating receptors, TNFα and IFNγ secretion and cytotoxic activity, however, treatment with IL-12 alone or in combinations with IL-15, IL-18 and IL-21 caused an increase in granzyme B and CD16 mRNA, IFNγ secretion and cytotoxic properties of the CNK-89 cell line.
我们建立了一个犬自然杀伤 (NK) 细胞系，称为 CNK-89，源自一只患有 NK 细胞肿瘤的狗。免疫表型分析显示 CD5、CD8、CD45、CD56、CD79a 和 NKp46 阳性染色，而 CD3、CD4、CD14、CD20、CD21、CD34、Thy1、IgG、IgM 和 MHCII 阴性染色。聚合酶链反应分析显示存在 CD56、NKG2D、NKp30、NKp44、NKp46 和穿孔素，但不存在 CD16、Ly49 和颗粒酶 B mRNA。用 IL-2 处理 CNK-89 细胞不会改变激活受体的表达、TNFα 和 IFNγ 的分泌和细胞毒性活性，但是单独用 IL-12 或与 IL-15、IL-18 和 IL-21 联合处理会导致 CNK-89 细胞系中颗粒酶 B 和 CD16 mRNA 的增加、IFNγ 的分泌和细胞毒性。
Zotero 插件