Shanxi Normal University, Linfen, Shanxi, 041000, China.
Shanxi Normal University, Linfen, Shanxi, 041000, China.
Anal Biochem. 2021 Jun 15;623:114171. doi: 10.1016/j.ab.2021.114171. Epub 2021 Mar 26.
Discrimination and detection of specific metal ions that belong to the same metallic element with different valence states in a complex matrix is challenging. In the present work, a fluorescence method using polyvinylpyrrolidone stabilized copper nanocluster (CuNCs@PVP) as a probe for discriminating detection of ferrous (Fe) and ferric (Fe) ions was developed. The CuNCs@PVP exhibited an excellent selective response to Fe ions in contrast to Fe ions and other metal ions when the pH value of solution was less than 4.0. Furthermore, the fluorescence of the CuNCs@PVP could be more sensitively quenched by Fe ions by virtue of Fenton reaction. The different response of CuNCs@PVP towards Fe and Fe ions under different conditions offered the potential for the discriminating detection of Fe and Fe ions. Based on detailed optimization of detection conditions, an excellent linear relationship between the fluorescence quenching efficiency (F/F) of the CuNCs@PVP and the concentration of Fe ions over the range of 0.4-20.0 μM and of Fe ions in the range of 0.01-0.4 μM were obtained, respectively. The detection limits for the Fe and Fe ions were 0.14 μM and 0.008 μM, respectively. The developed probe showed good selectivity and presented an alternative strategy for discriminating detection of Fe and Fe ions in complex samples.
在复杂基质中区分和检测属于同一金属元素但价态不同的特定金属离子具有挑战性。在本工作中，开发了一种使用聚乙烯吡咯烷酮稳定的铜纳米簇（CuNCs@PVP）作为探针用于区分检测亚铁（Fe）和铁（Fe）离子的荧光方法。当溶液的 pH 值小于 4.0 时，CuNCs@PVP 对 Fe 离子表现出优异的选择性响应，而对 Fe 离子和其他金属离子则没有。此外，CuNCs@PVP 的荧光可以通过 Fenton 反应更灵敏地被 Fe 离子猝灭。CuNCs@PVP 在不同条件下对 Fe 和 Fe 离子的不同响应为 Fe 和 Fe 离子的区分检测提供了潜力。基于检测条件的详细优化，CuNCs@PVP 的荧光猝灭效率（F/F）与 Fe 离子在 0.4-20.0 μM 范围内和 Fe 离子在 0.01-0.4 μM 范围内的浓度之间均获得了极好的线性关系。Fe 和 Fe 离子的检测限分别为 0.14 μM 和 0.008 μM。所开发的探针具有良好的选择性，并为复杂样品中 Fe 和 Fe 离子的区分检测提供了一种替代策略。
