You Lang, Li Fengxia, Sun Yan, Luo Liang, Qin Jian, Wang Tao, Liu Yuchen, Lai Ruogu, Li Ruohan, Guo Xiaoran, Mai Qiuyan, Pan Yihang, Xu Jianrong, Li Ningning
School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang, Sichuan, China.
Tomas Lindahl Nobel Laureate Laboratory, Precision Medicine Research Centre, The Seventh Affiliated Hospital of Sun Yat-sen University, Shenzhen, Guangdong, China.
Food Nutr Res. 2021 Mar 1;65. doi: 10.29219/fnr.v65.4246. eCollection 2021.
Obesity is a principal risk factor for the development of type 2 diabetes and cardiovascular diseases. Natural plants and/or foods play an important role in the management of obesity. (AAL) is a kind of potherb popular among Asian populations, and it is also consumed as a food ingredient and traditional herbal medicine.
We investigated the effects of water extract from AAL on high-fat-diet (HFD)-induced obese mice and 3T3-L1 adipocytes to develop a new functional food material.
Nine-week-old male mice were randomly divided into control (chow diet, = 6) and HFD ( = 30) group. From 12-weeks onward, mice in the HFD group were further separated into model (saline, 6 mL/kg), simvastatin (0.11 mg/mL, 6 mL/kg), and AAL treatment (low, middle, and high dosage: 300, 600, and 900 mg/kg) group, with 6 animals per group, while mice in the control group were treated with saline (6 mL/kg). Food intake, body/fat weight, liver/kidney indexes, and lipid profiles were determined. Tissues were fixed with formalin for pathological examination. Western blotting and PCR were performed to evaluate the protein and mRNA expression in 3T3-L1 adipocytes. Oil Red O staining was used to determine lipid accumulation.
AAL administration significantly suppressed body weight gain, and reduced fat pad weight and Lee's index in obese mice, but had no effect on liver/kidney index. AAL also reduced serum cholesterol, triglyceride, and LDL-C and increased HDL-C levels. Histological analysis revealed that AAL significantly ameliorated lipid accumulation in the liver and subcutaneous adipose tissue. , Oil Red O staining showed that AAL inhibited adipose differentiation by down-regulating the gene and protein expression of PPARγ and C/EBPα. AAL also reversed HFD-induced intestinal dysbacteriosis.
AAL water-soluble extract has a significant anti-adipogenic effect in the HFD-induced obese mice model.
肥胖是2型糖尿病和心血管疾病发生的主要危险因素。天然植物和/或食物在肥胖管理中发挥着重要作用。穿心莲（AAL）是一种在亚洲人群中很受欢迎的野菜，它也被用作食品成分和传统草药。
我们研究了穿心莲水提取物对高脂饮食（HFD）诱导的肥胖小鼠和3T3-L1脂肪细胞的影响，以开发一种新的功能性食品原料。
将9周龄雄性小鼠随机分为对照组（普通饮食，n = 6）和高脂饮食组（n = 30）。从12周起，高脂饮食组小鼠进一步分为模型组（生理盐水，6 mL/kg）、辛伐他汀组（0.11 mg/mL，6 mL/kg）和穿心莲治疗组（低、中、高剂量：300、600和900 mg/kg），每组6只动物，而对照组小鼠用生理盐水（6 mL/kg）处理。测定食物摄入量、体重/脂肪重量、肝脏/肾脏指数和血脂谱。组织用福尔马林固定用于病理检查。进行蛋白质免疫印迹和聚合酶链反应以评估3T3-L1脂肪细胞中的蛋白质和mRNA表达。用 Oil Red O 染色法测定脂质蓄积。
给予穿心莲显著抑制肥胖小鼠体重增加，降低脂肪垫重量和李氏指数，但对肝脏/肾脏指数无影响。穿心莲还降低了血清胆固醇、甘油三酯和低密度脂蛋白胆固醇水平，并提高了高密度脂蛋白胆固醇水平。组织学分析显示，穿心莲显著改善了肝脏和皮下脂肪组织中的脂质蓄积。此外，Oil Red O染色显示穿心莲通过下调PPARγ和C/EBPα的基因和蛋白质表达来抑制脂肪分化。穿心莲还逆转了高脂饮食诱导的肠道菌群失调。
穿心莲水溶性提取物在高脂饮食诱导的肥胖小鼠模型中具有显著的抗脂肪生成作用。
