Paprocki Jarosław, Sutkowy Paweł, Piechocki Jacek, Woźniak Alina
Department of Medical Biology and Biochemistry, Collegium Medicum of Nicolaus Copernicus University, Karłowicza 24, 85-092 Bydgoszcz, Poland.
Mazovian Centre for Hyperbaric Therapy and Wound Treatment in Warsaw, Wołoska 137, bud. "O", 02-507 Warszawa, Poland.
Oxid Med Cell Longev. 2021 Mar 10;2021:8895323. doi: 10.1155/2021/8895323. eCollection 2021.
The effect of vitamin D supplementation to patients with sudden sensorineural hearing loss (SSNHL), treated with hyperbaric oxygen (HBO) therapy, on the markers of the oxidant-antioxidant equilibrium was investigated. Patients were divided into two groups: those who did and did not receive vitamin D (cholecalciferol at 4000 IU/24 h). Concentrations of the following compounds, thiobarbituric acid reactive substances (TBARS), malondialdehyde (MDA), conjugated dienes (CD) in plasma and erythrocytes and activities of catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx) in erythrocytes, were determined. Haemoglobin (HGB) and haematocrit (HCT) were measured. Blood for analyses was collected from the basilic vein at three time points: before the first HBO procedure, up to 5 min after the first procedure, and after 14 procedures. No statistically significant differences in parameters tested were found between patients who did and did not receive vitamin D. In patients without supplementation, an increase of 53.2% ( ≤ 0.05) in erythrocyte TBARS was observed after the first HBO treatment. In patients receiving vitamin D, a reduction of 27.6% ( ≤ 0.05) was observed in erythrocyte MDA after 14 HBO treatments vs. that after the first treatment. In both groups, a decrease of 33.3% in plasma CD was observed after 14 treatments vs. that after the first treatment ( ≤ 0.05 and ≤ 0.01, respectively). No statistically significant changes were observed in the erythrocyte SOD, GPx, and CAT activities and in HCT. A reduction of HGB concentration of 10.9% ( ≤ 0.05) was demonstrated in nonsupplemented patients after 14 treatments compared with baseline. The results confirm that the effect of HBO therapy on oxidative stress markers is inconclusive and complex. Repeated HBO procedures can induce adaptive changes which protect against disruption of the oxidant-antioxidant equilibrium. It is possible that vitamin D supplementation inhibits the process of lipid peroxidation in erythrocytes.
研究了对接受高压氧（HBO）治疗的突发性感音神经性听力损失（SSNHL）患者补充维生素D对氧化-抗氧化平衡标志物的影响。患者分为两组：接受和未接受维生素D（胆钙化醇，4000 IU/24小时）的患者。测定了以下化合物的浓度：血浆和红细胞中的硫代巴比妥酸反应物质（TBARS）、丙二醛（MDA）、共轭二烯（CD），以及红细胞中过氧化氢酶（CAT）、超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GPx）的活性。测量了血红蛋白（HGB）和血细胞比容（HCT）。在三个时间点从贵要静脉采集分析用血：第一次HBO治疗前、第一次治疗后5分钟内以及14次治疗后。接受和未接受维生素D的患者之间，所测试的参数没有统计学上的显著差异。在未补充维生素D的患者中，第一次HBO治疗后红细胞TBARS增加了53.2%（P≤0.05）。在接受维生素D的患者中，14次HBO治疗后红细胞MDA较第一次治疗后降低了27.6%（P≤0.05）。两组中，14次治疗后血浆CD较第一次治疗后均降低了33.3%（分别为P≤0.05和P≤0.01）。红细胞SOD、GPx和CAT活性以及HCT没有观察到统计学上的显著变化。未补充维生素D的患者在14次治疗后与基线相比，HGB浓度降低了10.9%（P≤0.05）。结果证实，HBO治疗对氧化应激标志物的影响尚无定论且复杂。重复的HBO治疗可诱导适应性变化，防止氧化-抗氧化平衡的破坏。补充维生素D可能会抑制红细胞中的脂质过氧化过程。
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