Bader Nicolle, Bosy-Westphal Anja, Koch Andreas, Rimbach Gerald, Weimann Allan, Poulsen Henrik E, Müller Manfred J
Insititute of Biological Chemistry and Nutrition, University of Hohenheim, Garbenstrasse 28, D-70593, Stuttgart, Germany.
Br J Nutr. 2007 Oct;98(4):826-33. doi: 10.1017/S0007114507744380. Epub 2007 May 3.
The objectives of the present study were to evaluate the effect of normobaric and hyperbaric O2 (HBO) on plasma antioxidants and biomarkers of oxidative stress in plasma and urine and to investigate the effect of a 4-week vitamin C plus E supplementation on HBO-induced oxidative stress. Nineteen healthy men were exposed to HBO (100 % O2; 240 kPa) before and after 4 weeks' supplementation with 500 mg vitamin C plus 165 mg alpha-tocopherol equivalents. Exposure to 21 % O2 at 100 kPa served as intra-individual controls (control). Samples for the analysis of plasma antioxidants and oxidative stress biomarkers were collected before and immediately after each treatment. The present results showed that when compared with 'control', a single exposure to HBO resulted in a decrease of plasma vitamin C (P = 0.027) and an increase of lipid peroxides (P = 0.0008) and urinary 8-oxo-deoxyguanosine (8-oxodG) excretion (P = 0.006). Oxidative stress was not prevented by a 4-week supplementation with vitamins C and E. HBO-induced changes in plasma parameters correlated with basal antioxidant levels. The increase of urinary 8-oxodG after HBO plus supplementation correlated negatively with vitamin E intake (P = 0.023). We concluded that in healthy men HBO caused oxidative stress, which could not be prevented by dietary vitamin C plus E supplementation. The present data support the idea that HBO is a suitable model for oxidative stress in healthy volunteers.
本研究的目的是评估常压和高压氧（HBO）对血浆抗氧化剂以及血浆和尿液中氧化应激生物标志物的影响，并研究为期4周的维生素C加E补充剂对HBO诱导的氧化应激的影响。19名健康男性在补充500毫克维生素C加165毫克α-生育酚当量4周前后接受HBO（100%氧气；240千帕）治疗。在100千帕下暴露于21%氧气作为个体内对照（对照组）。在每次治疗前和治疗后立即采集用于分析血浆抗氧化剂和氧化应激生物标志物的样本。目前的结果表明，与“对照组”相比，单次暴露于HBO导致血浆维生素C降低（P = 0.027）、脂质过氧化物增加（P = 0.0008）以及尿8-氧代脱氧鸟苷（8-oxodG）排泄增加（P = 0.006）。为期4周的维生素C和E补充未能预防氧化应激。HBO诱导的血浆参数变化与基础抗氧化剂水平相关。HBO加补充剂后尿8-oxodG的增加与维生素E摄入量呈负相关（P = 0.023）。我们得出结论，在健康男性中，HBO会引起氧化应激，饮食中补充维生素C加E无法预防这种氧化应激。目前的数据支持HBO是健康志愿者氧化应激合适模型的观点。
