Department of Dermatology, The Second Affiliated Hospital of Kunming Medical University, Kunming, China.
Department of Burns, The Second Affiliated Hospital of Kunming Medical University, Kunming, China.
Diab Vasc Dis Res. 2021 Mar-Apr;18(2):14791641211002473. doi: 10.1177/14791641211002473.
The aim of the present study was to investigate the effect of the mobilization of EPCs by AMD3100 combined with G-CSF on wound healing in diabetic mice.
The full-thickness excisional wounds model of diabetic mice (db/db) was examined by hematoxylin and eosin staining, immunohistochemical staining, and western blotting to compare the wound healing and neovascularization among the combination, AMD3100 alone, G-CSF alone, and control groups.
The wounds reached the complete closure in the combination, AMD3100 alone, G-CSF alone, and control groups on days 17, 20, 21, 21 after surgery, respectively. In addition, the combination group promoted the inflammatory cell recruitment and glandular formation. On day 10 from injury, the protein expression of CD31 in the combination group was significantly higher compared with the other three groups ( < 0.001). The level of SDF-1 protein remained high in the combined group until on day 10 after surgery ( < 0.001).
The mobilization of endogenous EPCs by AMD3100 combine with G-CSF is able to enhance the complete healing of full-thickness wounds and neovascularization in db/db mice may by SDF-1/CXCR4 axis. These findings provided a novel method and indication of duration of mobilization on diabetic wound healing and tissue regeneration.
本研究旨在探讨 AMD3100 联合 G-CSF 动员 EPCs 对糖尿病小鼠伤口愈合的影响。
通过苏木精-伊红染色、免疫组织化学染色和 Western blot 分析，研究糖尿病小鼠（db/db）的全层切除创面模型，比较联合组、AMD3100 组、G-CSF 组和对照组的创面愈合和新生血管化情况。
术后第 17、20、21 和 21 天，联合组、AMD3100 组、G-CSF 组和对照组的创面均完全闭合。此外，联合组促进了炎性细胞募集和腺形成。伤后第 10 天，联合组 CD31 蛋白表达明显高于其他三组（<0.001）。联合组 SDF-1 蛋白水平在术后第 10 天仍保持较高水平（<0.001）。
AMD3100 联合 G-CSF 动员内源性 EPCs 能够增强 db/db 小鼠全层创面的完全愈合和新生血管化，可能通过 SDF-1/CXCR4 轴实现。这些发现为糖尿病创面愈合和组织再生提供了一种新的方法和动员时间的指示。
