Watson W S, Finlay A Y
Department of Clinical Physics and Bioengineering, Southern General Hospital, Glasgow, U.K.
Br J Dermatol. 1988 Apr;118(4):523-30. doi: 10.1111/j.1365-2133.1988.tb02462.x.
The multiple skin surface biopsy technique to measure stratum corneum (SC) penetration characteristics in vivo has been more accurately quantified and used to provide information on the kinetics of transfer of a topical steroid through the SC. Radiolabelled clobetasol 17-propionate formulated in two different vehicles, consisting primarily of dibutyl adipate or of propylene glycol, was applied and the penetration characteristics compared. Using exponential expressions for radioactivity in skin surface biopsies as a function of SC depth, and total SC radioactivity as a function of time, a mathematical model was developed to calculate the rate of loss of steroid through superficial shedding and the rate of transfer of steroid from the SC into the epidermis. When a topical steroid was applied for 8 h under gauze occlusion, over 80% was recovered from the gauze.
用于在体内测量角质层（SC）渗透特性的多皮肤表面活检技术已得到更精确的量化，并用于提供局部类固醇通过SC的转运动力学信息。将主要由己二酸二丁酯或丙二醇组成的两种不同载体中配制的放射性标记的丙酸氯倍他索进行应用，并比较其渗透特性。利用皮肤表面活检中放射性作为SC深度的函数的指数表达式，以及总SC放射性作为时间的函数，建立了一个数学模型，以计算类固醇通过表面脱落的损失率和类固醇从SC向表皮的转运率。当在纱布封闭下应用局部类固醇8小时时，超过80%从纱布中回收。
