Wahdan Sara Fareed Mohamed, Reitz Thomas, Heintz-Buschart Anna, Schädler Martin, Roscher Christiane, Breitkreuz Claudia, Schnabel Beatrix, Purahong Witoon, Buscot François
Department of Soil Ecology, UFZ-Helmholtz Centre for Environmental Research, Theodor-Lieser-Str. 4, Halle (Saale), 06120, Germany.
Department of Biology, Leipzig University, Johannisallee 21, Leipzig, 04103, Germany.
Environ Microbiol. 2021 Oct;23(10):6163-6176. doi: 10.1111/1462-2920.15492. Epub 2021 Apr 10.
Climate and agricultural practice interact to influence both crop production and soil microbes in agroecosystems. Here, we carried out a unique experiment in Central Germany to simultaneously investigate the effects of climates (ambient climate vs. future climate expected in 50-70 years), agricultural practices (conventional vs. organic farming), and their interaction on arbuscular mycorrhizal fungi (AMF) inside wheat (Triticum aestivum L.) roots. AMF communities were characterized using Illumina sequencing of 18S rRNA gene amplicons. We showed that climatic conditions and agricultural practices significantly altered total AMF community composition. Conventional farming significantly affected the AMF community and caused a decline in AMF richness. Factors shaping AMF community composition and richness at family level differed greatly among Glomeraceae, Gigasporaceae and Diversisporaceae. An interactive impact of climate and agricultural practices was detected in the community composition of Diversisporaceae. Organic farming mitigated the negative effect of future climate and promoted total AMF and Gigasporaceae richness. AMF richness was significantly linked with nutrient content of wheat grains under both agricultural practices.
气候与农业实践相互作用，影响着农业生态系统中的作物产量和土壤微生物。在此，我们在德国中部开展了一项独特的实验，以同时研究气候（当前气候与预计50 - 70年后的未来气候）、农业实践（传统农业与有机农业）及其相互作用对小麦（Triticum aestivum L.）根系内丛枝菌根真菌（AMF）的影响。利用18S rRNA基因扩增子的Illumina测序对AMF群落进行了表征。我们发现，气候条件和农业实践显著改变了AMF群落的总体组成。传统农业显著影响了AMF群落，并导致AMF丰富度下降。在球囊霉科、巨孢囊霉科和多样孢囊霉科中，影响AMF群落组成和丰富度的因素在科水平上差异很大。在多样孢囊霉科的群落组成中检测到气候与农业实践的交互影响。有机农业减轻了未来气候的负面影响，并提高了AMF和巨孢囊霉科的总体丰富度。在两种农业实践下，AMF丰富度均与小麦籽粒的养分含量显著相关。
