Zhang Xueju, Wu Chuanbin, Tan Wen
Postdoctoral Innovation Base, Zhuhai Yuanzhi Health Technology Co. Ltd, Hengqin New Area, Zhuhai, Guangdong 519000, China.
College of Pharmacy, Jinan University, Guangzhou, Guangdong 510632, China.
J Proteome Res. 2021 May 7;20(5):2643-2650. doi: 10.1021/acs.jproteome.0c01050. Epub 2021 Mar 29.
Alzheimer's disease (AD) is closely associated with lipid metabolism dysfunction. However, space distribution and metabolism of aberrant lipids in the brain of early-stage AD mouse remain unclear. In our current work, a novel lipidomics method based on mass spectrometry imaging was developed to visually disclose molecular perturbation and characterize space distribution in the brain of double transgenic amyloid precursor protein/presenilin 1 mouse (2 and 3 months old). Significant changes were detected, including phosphatidylethanolamines, phosphatidylcholines, fatty acids, lysophospholipids, and glycerides in AD mouse brain. The results in this study suggest that these significantly altered lipid metabolic pathways (glycerophospholipid metabolism) may be implicated in early-stage AD. Our work deepens the understanding of the physio-pathologic mechanism of early-stage AD.
阿尔茨海默病（AD）与脂质代谢功能障碍密切相关。然而，早期AD小鼠大脑中异常脂质的空间分布和代谢仍不清楚。在我们目前的工作中，开发了一种基于质谱成像的新型脂质组学方法，以直观地揭示双转基因淀粉样前体蛋白/早老素1小鼠（2个月和3个月大）大脑中的分子扰动并表征其空间分布。检测到显著变化，包括AD小鼠大脑中的磷脂酰乙醇胺、磷脂酰胆碱、脂肪酸、溶血磷脂和甘油酯。本研究结果表明，这些显著改变的脂质代谢途径（甘油磷脂代谢）可能与早期AD有关。我们的工作加深了对早期AD生理病理机制的理解。
