Okulicz W C, Darrow J M, Goldman B D
Worcester Foundation for Experimental Biology, Shrewsbury, Massachusetts 01545.
Biol Reprod. 1988 Apr;38(3):597-604. doi: 10.1095/biolreprod38.3.597.
The Turkish hamster is a long-day breeder that hibernates for 4-5 mo if exposed to a short-day, cold environment. The objective of this study was to assess the uterine responsiveness of the hibernating animal to ovarian steroids. Our approach was 1) to characterize and determine uterine estrogen (E) and progesterone (P) receptors (R) during hibernation as compared to the levels observed in cycling females that had terminated hibernation, and 2) to assess the responsiveness of the uterus to E during hibernation by its ability to induce uterine P receptor. Females were exposed to short days (10L:14D) for 2 mo and then were placed in a cold-room (10L: 14D, 6 +/- 1 degrees C). After 2 or 4 mo in the cold, hibernating animals were killed and uterine steroid receptors were determined by 3H-steroid binding assay. Uterine receptors were also determined in cycling Turkish hamsters on each morning of the estrous cycle. Values for uterine receptors (pmol/g tissue, n = 4-6) during the estrous cycle (estrus, diestrus I, diestrus II, proestrus) were: 4.3 +/- 0.78, 3.9 +/- 0.19, 4.1 +/- 0.25, 3.7 +/- 0.5 for cytosolic ER; 36.6 +/- 5.8, 32.2 +/- 6.8, 36.3 +/- 1.5, 54.4 +/- 1.9 for cytosolic PR; 0.59 +/- 0.11, 0.54 +/- 0.07, 1.06 +/- 0.05, 1.42 +/- 0.17 for nuclear ER. Hibernating (torpid) animals sampled after 2 mo in the cold showed a significant (p less than 0.05) depression of cytosolic ER (2.6 +/- 0.12, n = 5) and cytosolic PR (19.0 +/- 2.6, n = 8) as compared to any day of the estrous cycle.(ABSTRACT TRUNCATED AT 250 WORDS)
土耳其仓鼠是一种长日照繁殖动物，如果处于短日照、寒冷环境中，会冬眠4 - 5个月。本研究的目的是评估冬眠动物子宫对卵巢类固醇的反应性。我们的方法是：1）与已结束冬眠的处于发情周期的雌性仓鼠相比，对冬眠期间子宫雌激素（E）和孕激素（P）受体（R）进行特征描述并测定其水平；2）通过诱导子宫P受体的能力来评估冬眠期间子宫对E的反应性。将雌性仓鼠置于短日照（10小时光照：14小时黑暗）环境中2个月，然后放入冷藏室（10小时光照：14小时黑暗，6±1℃）。在寒冷环境中2个月或4个月后，处死冬眠动物，通过³H - 类固醇结合试验测定子宫类固醇受体。在发情周期的每个早晨，也对处于发情周期的土耳其仓鼠的子宫受体进行测定。发情周期（发情期、动情间期I、动情间期II、发情前期）期间子宫受体的值（pmol/g组织，n = 4 - 6）为：胞质雌激素受体分别为4.3±0.78、3.9±0.19、4.1±0.25、3.7±0.5；胞质孕激素受体分别为36.6±5.8、32.2±6.有帮助，谢谢！8、36.3±1.5、54.4±1.9；核雌激素受体分别为）与发情周期的任何一天相比，在寒冷环境中2个月后取样的冬眠（蛰伏）动物的胞质雌激素受体（2.6±0.12，n = 5）和胞质孕激素受体（19.0±2.6，n = 8）显著降低（p＜0.05）。（摘要截断于250字） 0.59±0.11、0.54±0.07、1.06±0.05、1.42±0.17。
