State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Industrial Fermentation Microbiology, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, China; College of Public Health, Zunyi Medical University, Zunyi 563000, China.
State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Industrial Fermentation Microbiology, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, China.
Food Chem. 2021 Sep 1;355:129444. doi: 10.1016/j.foodchem.2021.129444. Epub 2021 Mar 3.
Melanoidins, are of increasing interest for their potential biological activities. However, little knowledge is available on their structure. In the present study, vinegar, coffee and model melanoidins were degraded by NaBH, and the resultant reaction products were characterised by chromatography, mass spectrometry and spectrometry methods to elucidate the mechanism of formation of melanoidin skeleton molecules. The study identified a typical polymer with a molecular weight (MW) interval of 74 Da, which was polymerised by aldol condensation and reduced by NaBH, followed by intermolecular dehydration. MW of the theoretically derived typical polymers matched the detected polymers, validating the speculated pathway involved in the formation of melanoidins skeleton molecules. The study also revealed that melanoidins from different sources contain polymers with the same MW and different binding preferences, contributing to the heterogeneity of melanoidins. Overall, these findings indicated that the identified polymers could be used as potential candidate biomarkers for melanoidins.
类黑素因其潜在的生物活性而受到越来越多的关注。然而，关于它们的结构知之甚少。本研究采用 NaBH4 降解醋、咖啡和模型类黑素，通过色谱、质谱和光谱方法对反应产物进行了表征，以阐明类黑素骨架分子的形成机制。研究发现了一种典型的聚合物，其分子量（MW）间隔为 74Da，它通过醛醇缩合聚合，然后由 NaBH4 还原，再进行分子间脱水。理论推导的典型聚合物的 MW 与检测到的聚合物相匹配，验证了推测的类黑素骨架分子形成途径。研究还表明，来自不同来源的类黑素含有具有相同 MW 但结合偏好不同的聚合物，这导致了类黑素的异质性。总的来说，这些发现表明，所鉴定的聚合物可用作类黑素的潜在候选生物标志物。
