Department of Civil Engineering, Indian Institute of Technology, Guwahati, Assam, 781039, India.
Department of Civil Engineering, Indian Institute of Technology, Guwahati, Assam, 781039, India.
J Environ Manage. 2021 Jun 15;288:112430. doi: 10.1016/j.jenvman.2021.112430. Epub 2021 Mar 27.
The present study deals with the biotransformation of virulent petroleum refinery concoction with phenol (750 mg/L), emulsified crude oil (300 mg/L), S (750 mg/L), NH-N (350 mg/L) and NO-N (1000 mg/L) in anoxic (A1) - aerobic (A2) moving bed reactors operated in series. The efficacy of the system was analysed through measurement of pollutant concentrations, GC-MS and FTIR peaks of the influent and effluent, and biomass activity studies. The system was able to eliminate the organics and inorganics with more than 99% efficiency at 80 h HRT and 64 h cycle time. GC-MS results revealed breakage of high molecular weight organics to smaller compounds after anoxic treatment. Further treatment of anoxic effluent by aerobic biomass reduced the number of peaks in the final effluent significantly. FTIR results were in accord with the GC-MS results. Heterotrophic activity (HA) of the aerobic biomass was higher than anoxic biomass due to its higher free energy change. Anoxic biomass showed chemolithotrophic activity (CA), suggesting survival in the absence of organics. Gas generated from anoxic reactor consisted of 91% nitrogen, 1% CO, 1% HS and rest was unaccounted.
本研究涉及用生物转化方法处理含酚（750mg/L）、乳化原油（300mg/L）、S（750mg/L）、NH-N（350mg/L）和 NO-N（1000mg/L）的有毒石油精炼混合物，在串联运行的缺氧（A1）-好氧（A2）移动床反应器中进行。通过测量进水和出水的污染物浓度、GC-MS 和 FTIR 峰以及生物量活性研究来分析该系统的效果。该系统在 80h 的 HRT 和 64h 的循环时间下，能够以超过 99%的效率去除有机物和无机物。GC-MS 结果表明，缺氧处理后，高分子有机物断裂成较小的化合物。进一步用好氧生物量处理缺氧出水，可使最终出水中的峰数显著减少。FTIR 结果与 GC-MS 结果一致。由于好氧生物量的自由能变化较高，其异养活性（HA）高于缺氧生物量。缺氧生物量表现出化能自养活性（CA），表明其在没有有机物的情况下能够存活。缺氧反应器中产生的气体由 91%的氮、1%的 CO、1%的 HS 和其余未被记录的气体组成。
