Moreno-Ayala Roberto, Junker Jan Philipp
Brief Funct Genomics. 2021 Mar 30. doi: 10.1093/bfgp/elab018.
New developments in single-cell genomics have transformed developmental biology in recent years by enabling systematic analysis of embryonic cell types and differentiation trajectories. Ongoing efforts in experimental and computational method development aim to reveal gene-regulatory mechanisms and to provide additional spatio-temporal information about developmental cell fate decisions. Here, we discuss recent technological developments as well as biological applications of single-cell genomics, with a particular focus on analysis of developmental cell fate decisions. Although the approaches described here are generally applicable to a broad range of model systems, we focus our discussion on applications in zebrafish, which has proven to be a particularly powerful model organism for establishing novel methods in single-cell genomics.
近年来，单细胞基因组学的新进展通过对胚胎细胞类型和分化轨迹进行系统分析，改变了发育生物学。在实验和计算方法开发方面的持续努力旨在揭示基因调控机制，并提供有关发育细胞命运决定的更多时空信息。在这里，我们讨论单细胞基因组学的最新技术进展以及生物学应用，特别关注发育细胞命运决定的分析。尽管这里描述的方法通常适用于广泛的模型系统，但我们将讨论重点放在斑马鱼中的应用上，事实证明斑马鱼是在单细胞基因组学中建立新方法的特别强大的模式生物。
