Kalina M, Riklis S
Department of Histology and Cell Biology, Sackler School of Medicine, Tel Aviv University, Israel.
Cell Differ. 1988 Apr;23(3):231-6. doi: 10.1016/0045-6039(88)90076-0.
Alveolar type II-like colonies were obtained after a low density plating (5 X 10(3)/60 mm tissue culture dish) of primary type II cells. These colonies were formed only when type II cells were either cocultured with alveolar macrophages or with conditioned media generated by alveolar macrophages. Cells in the colonies appeared homogeneous and kept their lamellar bodies over a period of 8 weeks and more, as observed by electron microscopy. These cells reacted immunocytochemically with antibodies directed against the 32-38 kDa protein fractions of rat surfactant.
在对原代II型细胞进行低密度接种（5×10³个细胞/60mm组织培养皿）后，获得了II型肺泡样集落。这些集落仅在II型细胞与肺泡巨噬细胞共培养或与肺泡巨噬细胞产生的条件培养基共培养时形成。通过电子显微镜观察，集落中的细胞看起来均匀一致，并且在8周及更长时间内都保持其板层小体。这些细胞与针对大鼠表面活性剂32 - 38kDa蛋白组分的抗体发生免疫细胞化学反应。
