Zheng Xiaomei, Zheng Ping, Sun Jibin
Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China.
Key Laboratory of Systems Microbial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China.
Sheng Wu Gong Cheng Xue Bao. 2021 Mar 25;37(3):980-990. doi: 10.13345/j.cjb.200613.
Aspergillus niger is a vital industrial workhouse widely used for the production of organic acids and industrial enzymes. This fungus is a crucial cell factory due to its innate tolerance to a diverse range of abiotic conditions, high production titres, robust growth during industrial scale fermentation, and status as a generally recognized as safe (GRAS) organism. Rapid development of synthetic biology and systems biology not only offer powerful approaches to unveil the molecular mechanisms of A. niger productivity, but also provide more new strategies to construct and optimize the A. niger cell factory. As a new generation of genome editing technology, the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR associated (Cas) system brings a revolutionary breakthrough in targeted genome modification for A. niger. In this review, we focus on current advances to the CRISPR/Cas genome editing toolbox, its application on gene modification and gene expression regulation in this fungal. Moreover, the future directions of CRISPR/Cas genome editing in A. niger are highlighted.
黑曲霉是一种重要的工业微生物，广泛用于生产有机酸和工业酶。由于其对多种非生物条件具有天然耐受性、高产率、在工业规模发酵过程中生长旺盛以及被普遍认为是安全（GRAS）的生物体，这种真菌是一种关键的细胞工厂。合成生物学和系统生物学的快速发展不仅为揭示黑曲霉生产能力的分子机制提供了有力方法，还为构建和优化黑曲霉细胞工厂提供了更多新策略。作为新一代基因组编辑技术，成簇规律间隔短回文重复序列（CRISPR）/CRISPR相关（Cas）系统在黑曲霉的靶向基因组修饰方面带来了革命性突破。在这篇综述中，我们重点关注CRISPR/Cas基因组编辑工具箱的当前进展、其在该真菌中的基因修饰和基因表达调控方面的应用。此外，还强调了黑曲霉中CRISPR/Cas基因组编辑的未来方向。
