Liu Guodong, Gao Liwei, Qu Yinbo
State Key Laboratory of Microbial Technology, National Glycoengineering Research Center, Shandong University, Qingdao 266237, Shandong, China.
Sheng Wu Gong Cheng Xue Bao. 2021 Mar 25;37(3):1058-1069. doi: 10.13345/j.cjb.200531.
The efficient production of lignocellulolytic enzyme systems is an important support for large-scale biorefinery of plant biomass. On-site production of lignocellulolytic enzymes could increase the economic benefits of the process by lowering the cost of enzyme usage. Penicillium species are commonly found lignocellulose-degrading fungi in nature, and have been used for industrial production of cellulase preparations due to their abilities to secrete complete and well-balanced lignocellulolytic enzyme systems. Here, we introduce the reported Penicillium species for cellulase production, summarize the characteristics of their enzymes, and describe the strategies of strain engineering for improving the production and performance of lignocellulolytic enzymes. We also review the progress in fermentation process optimization regarding the on-site production of lignocellulolytic enzymes using Penicillium species, and suggest prospect of future work from the perspective of building a "sugar platform" for the biorefinery of lignocellulosic biomass.
高效生产木质纤维素酶系统是植物生物质大规模生物炼制的重要支撑。木质纤维素酶的现场生产可通过降低酶使用成本来提高该过程的经济效益。青霉属真菌是自然界中常见的木质纤维素降解真菌,由于其能够分泌完整且平衡良好的木质纤维素酶系统,已被用于纤维素酶制剂的工业生产。在此,我们介绍已报道的用于生产纤维素酶的青霉属真菌,总结其酶的特性,并描述用于提高木质纤维素酶生产和性能的菌株工程策略。我们还综述了利用青霉属真菌进行木质纤维素酶现场生产的发酵过程优化进展,并从构建木质纤维素生物质生物炼制“糖平台”的角度提出未来工作展望。