Shen Ying, Hu Tian-Jue, Zeng Guang-Ming, Huang Dan-Lian, Yin Lu, Liu Yang, Wu Juan-Juan, Liu Hui
College of Environmental Science and Engineering, Hunan University, Changsha 410082, China.
Huan Jing Ke Xue. 2013 Feb;34(2):781-8.
Penicillium simplicissimum(Oudem.) Thomrn BGA can secrete lignocellulolytic enzymes, among these enzymes the highest activities of hemicellulase, cellulase, lignin peroxidase (Lip), manganese peroxidase (Mnp) and laccase are 146.82 Iu.g-1, 2.78 U.g-1, 47.97 U.g-1, 34.56 U.g-1 and 17.94 U.g-1 respectively. According to the results and the statistical analysis of SPSS, the ability of secreting lignocellulolytic enzymes by Penicillium simplicissimum significantly correlated with the structure of lignocellulose, and the biodegradation of lignocellulose was probably a kind of synergistic effect of several lignocellulolytic enzymes. In the solid-state fermentation of 30 days, the hemicellulose content has a significantly negative correlation with the fermentation days(r = -0.946, P < 0.01), there was also a significantly negative correlation between the cellulose-biodegradation and the lignin-biodegradation (r = -0.818, P<0.05). As unselected enzymes, Lip and Mnp can degrade hemicellulose and cellulose corporately when biodegrades lignin. The significant correlation is showed between Lip, Mnp and cellulose (correlation parameters are r = 0. 922, P <0.01; r = 0.807, P<0.05 respectively). In addition, the biosorption is found to have a very important effect in the removal of liquid alkali lignin by Penicillium simplicissimum. Key words:Penicillium simplicissimum; lignocellulolytic enzymes; biodegradation; biosorption; synergistic effect
简单青霉（Oudem.）Thomrn BGA能够分泌木质纤维素分解酶，在这些酶中，半纤维素酶、纤维素酶、木质素过氧化物酶（Lip）、锰过氧化物酶（Mnp）和漆酶的最高活性分别为146.82 Iu.g-1、2.78 U.g-1、47.97 U.g-1、34.56 U.g-1和17.94 U.g-1。根据结果以及SPSS统计分析，简单青霉分泌木质纤维素分解酶的能力与木质纤维素结构显著相关，木质纤维素的生物降解可能是几种木质纤维素分解酶的协同作用。在30天的固态发酵中，半纤维素含量与发酵天数呈显著负相关（r = -0.946，P < 0.01），纤维素生物降解与木质素生物降解之间也存在显著负相关（r = -0.818，P<0.05）。作为未被选择的酶，Lip和Mnp在降解木质素时可共同降解半纤维素和纤维素。Lip、Mnp与纤维素之间呈现显著相关性（相关参数分别为r = 0. 922，P <0.01；r = 0.807，P<0.05）。此外，发现生物吸附在简单青霉去除液体碱木质素方面具有非常重要的作用。关键词：简单青霉；木质纤维素分解酶；生物降解；生物吸附；协同效应
