Katagiri Wataru, Endo Satoshi, Takeuchi Ryoko, Suda Daisuke, Saito Naoaki, Kobayashi Tadaharu
Division of Reconstructive Surgery for Oral and Maxillofacial Region, Faculty of Dentistry & Graduate School of Medical and Dental Science, Niigata University, 2-5274 Gakkocho-dori, Chuo-ku, Niigata, 951-8514, Japan.
Heliyon. 2021 Mar 16;7(3):e06530. doi: 10.1016/j.heliyon.2021.e06530. eCollection 2021 Mar.
Condylar resorption (CR) after surgical orthognathic treatment is defined as dysfunctional remodeling of the temporomandibular joint manifested by morphological changes with decreased condylar head volume that cause occlusal and esthetic changes. Although both conservative and surgical treatment strategies have been employed for the treatment of CR, effective procedures have not been established till date. In this study, the effects of MSC-CM on CR were investigated. Bone marrow-derived MSCs of rats (rMSCs) were cultured until 80% confluent, cultured in serum-free conditioned medium for 48 h; the collected medium was defined as MSC-CM. Osteogenesis, chondrogenesis, and angiogenesis-related gene expression in rMSCs cultured with MSC-CM was evaluated by quantitative real-time polymerase chain reaction. A rat CR model was used for animal studies, in which CR occurred after mandibular distraction osteogenesis for 10 days. MSC-CM was injected via the tail vein and quantitative and qualitative evaluations were performed by micro-computed tomography (micro-CT) and histology. MSC-CM enhanced osteogenesis-, chondrogenesis-, and angiogenesis-related gene expression in rMSCs. Micro-CT showed CR in control groups; however, it was observed to be improved in the MSC-CM group. Histologically, an enlarged cartilage layer was seen in the MSC-CM group, while cartilage layers had almost thinned or disappeared in control groups. These results indicate that MSC-CM improved CR.
外科正颌治疗后髁突吸收（CR）被定义为颞下颌关节的功能失调性重塑，表现为形态学改变，髁突头部体积减小，导致咬合和美观变化。尽管已经采用了保守和手术治疗策略来治疗CR，但迄今为止尚未确立有效的治疗方法。在本研究中，研究了间充质干细胞条件培养基（MSC-CM）对CR的影响。将大鼠骨髓来源的间充质干细胞（rMSCs）培养至80%汇合，在无血清条件培养基中培养48小时；收集的培养基定义为MSC-CM。通过定量实时聚合酶链反应评估用MSC-CM培养的rMSCs中成骨、软骨生成和血管生成相关基因的表达。使用大鼠CR模型进行动物研究，其中在进行10天的下颌骨牵张成骨后发生CR。通过尾静脉注射MSC-CM，并通过微型计算机断层扫描（micro-CT）和组织学进行定量和定性评估。MSC-CM增强了rMSCs中成骨、软骨生成和血管生成相关基因的表达。Micro-CT显示对照组有CR；然而，在MSC-CM组中观察到有所改善。组织学上，MSC-CM组可见软骨层增厚，而对照组软骨层几乎变薄或消失。这些结果表明MSC-CM改善了CR。
