使用流式细胞术检测细胞周期状态。
Assaying Cell Cycle Status Using Flow Cytometry.
机构信息
Department of Oncological Sciences, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
出版信息
Methods Mol Biol. 2021;2267:165-179. doi: 10.1007/978-1-0716-1217-0_11.
In this chapter, four methods are described that can be used to assess cell cycle status in flow cytometry. The first method is based on the simultaneous analysis of cellular DNA content using a fluorescent DNA dye (propidium iodide) and of a nuclear proliferation marker (Ki-67). The second is based on the differential staining of DNA and RNA using Hoechst 33342 and Pyronin Y: this method is particularly useful to distinguish quiescent cells in G0 phase from G1 cells. Finally, two methods are described based on DNA incorporation of the synthetic nucleosides BrdU and EdU.
在本章中，描述了四种可用于流式细胞术评估细胞周期状态的方法。第一种方法基于使用荧光 DNA 染料（碘化丙啶）和核增殖标志物（Ki-67）同时分析细胞 DNA 含量。第二种方法基于使用 Hoechst 33342 和 Pyr onin Y 对 DNA 和 RNA 进行差异染色：这种方法特别有助于区分 G0 期的静止细胞和 G1 细胞。最后，描述了两种基于合成核苷 BrdU 和 EdU 的 DNA 掺入的方法。
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