Olowofolahan Adeola Oluwakemi, Adewoye Funmilayo O, Olorunsogo Olufunso Olabode
Department of Biochemistry, Faculty of Basic Medical Sciences, College of Medicine, University of Ibadan, Ibadan, Nigeria.
J Complement Integr Med. 2021 Mar 31;18(2):355-361. doi: 10.1515/jcim-2019-0341.
Uterine fibroids are benign tumors that develop in many women of reproductive age. Surgery is the main approach to treatment while other options are also associated with adverse effects. Studies have shown that certain bioactive agents present in medicinal plants elicit their anti-tumor activity by induction of mitochondrial permeability transition (mPT) opening. This research therefore aimed at investigating the effect of methanol extract of (MEAM) on mPT pore opening in normal and monosodium glutamate-induced uterine hyperplasia using female Wistar rats.
Mitochondria, isolated from rat liver were exposed to different concentrations (20, 60, 100, 140 and 180 μg/mL) of MEAM. The mPT pore opening, cytochrome release, mitochondrial ATPase (mATPase) activity and the percentage lipid peroxidation were assessed spectrophotometrically. Histological effects of MEAM on the liver, brain and uterus of normal and MSG-treated rats were investigated.
The results showed a significant induction of mPT pore opening by 2.4, 4.2 and 6.4 folds, release of cytochrome and enhancement of mATPase activity at 100,140 and 180 μg/mL, respectively. However, oral administration of MEAM did not induce mPT pore opening, neither any significant release of cytochrome nor enhancement of mATPase activity at all the dosages used. However, histological assay revealed the presence of MSG-induced cellular damage and uterine hyperplasia which was ameliorated by MEAM co-administration.
These findings suggest that MEAM contains phytochemicals that can ameliorate MSG-induced damage and uterine hyperplasia in rats; however, the mechanism might not be via upregulation of mitochondrial-mediated apoptosis.
子宫肌瘤是一种发生于许多育龄女性的良性肿瘤。手术是主要的治疗方法，而其他治疗选择也会带来不良反应。研究表明，药用植物中存在的某些生物活性物质通过诱导线粒体通透性转换（mPT）孔开放发挥其抗肿瘤活性。因此，本研究旨在探讨[植物名称]甲醇提取物（MEAM）对雌性Wistar大鼠正常及味精诱导的子宫增生中mPT孔开放的影响。
将从大鼠肝脏分离的线粒体暴露于不同浓度（20、60、100、140和180μg/mL）的MEAM中。采用分光光度法评估mPT孔开放、细胞色素释放、线粒体ATP酶（mATPase）活性及脂质过氧化百分比。研究了MEAM对正常及味精处理大鼠的肝脏、脑和子宫的组织学影响。
结果显示，在100、140和180μg/mL时，mPT孔开放分别显著诱导2.4倍、4.2倍和6.4倍，细胞色素释放及mATPase活性增强。然而，口服MEAM在所有使用剂量下均未诱导mPT孔开放，也未引起细胞色素的任何显著释放或mATPase活性增强。不过，组织学分析显示存在味精诱导的细胞损伤和子宫增生，而MEAM联合给药可改善这种情况。
这些发现表明，MEAM含有可改善大鼠味精诱导的损伤和子宫增生的植物化学物质；然而，其机制可能不是通过上调线粒体介导的细胞凋亡。
