Academic Unit of Garanhuns, Federal Rural University of Pernambuco, Garanhuns, Brazil.
Laboratory of Basic Biology Teaching Azarias Salgado/LABAS, Reference High School Azarias Salgado, Angelim, Brazil.
Prep Biochem Biotechnol. 2022;52(1):30-37. doi: 10.1080/10826068.2021.1904257. Epub 2021 Mar 31.
The present study evaluated the influence of the variables polyethylene glycol (PEG) molar mass, pH, PEG concentration and sodium citrate concentration in the integrated production of the protease from Kita UCP1279 by extractive fermentation, obtaining as a response the partition coefficient (), activity yield () and concentration factor (). The enzyme preferably partitioned to the top phase and obtained in the system formed by variables M = 400 g mol, C = 20% (w w), and C = 20% (w w) and pH 6, in this condition were obtained = 1.90 and = 79.90%. The protease showed stability at a temperature of 60 °C for 180 min, with optimum temperature 40 °C and pH 8.0. For the ions and inhibitors effects, the protease activity increased when exposed to Fe, Ca and Zn and inhibited by EDTA, being classified as metalloprotease. The kinetic parameters (35.63 mg mL) and (1.205 mg mLmin) were also estimated. Thus, the protease showed desirable characteristics that enable future industrial applications, especially, for beer industry.
本研究评估了变量聚乙二醇（PEG）分子量、pH 值、PEG 浓度和柠檬酸钠浓度对从 Kita UCP1279 通过萃取发酵集成生产蛋白酶的影响，得到分配系数（）、活性产率（）和浓缩因子（）作为响应。该酶优先分配到上相，并在由变量 M = 400 g mol、C = 20%（w w）和 C = 20%（w w）和 pH 6 组成的系统中获得，在这种条件下，获得了 = 1.90 和 = 79.90%。该蛋白酶在 60°C 下 180 分钟内表现出稳定性，最适温度为 40°C，pH 值为 8.0。对于离子和抑制剂的影响，蛋白酶的活性在暴露于 Fe、Ca 和 Zn 时增加，而被 EDTA 抑制，被归类为金属蛋白酶。还估计了动力学参数（35.63 mg mL）和（1.205 mg mLmin）。因此，该蛋白酶表现出理想的特性，使其能够在未来的工业应用中得到应用，特别是在啤酒工业中。
