Universidade Estadual Paulista - UNESP, Faculdade de Ciências Agronômicas, Departamento de Proteção Vegetal, Botucatu, SP, Brasil.
University of Florida, Gulf Coast Research and Education Center, Wimauma, FL, USA.
Braz J Biol. 2021 Mar 26;82:e237098. doi: 10.1590/1519-6984.237098. eCollection 2021.
Endosymbiont bacteria can affect biological parameters and reduce the effectiveness of natural enemies in controlling the target insect. The objective of this work was to identify endosymbiont bacteria in Anaphes nitens (Girault, 1928) (Hymenoptera: Mymaridae), the main natural enemy used to manage Gonipterus platensis (Marelli, 1926) (Coleoptera: Curculionidae). Genomic DNA from six A. nitens populations was extracted and polymerase chain reactions (PCR) were performed with the primers to detect endosymbiont bacteria in this insect. The PCR products were amplified, sequenced, and compared with sequences deposited in the GenBank for the bacteria identification. All A. nitens populations had the bacterium Yersinia massiliensis (Enterobacteriales: Enterobacteriaceae). This bacterium was originally described as free-living, and it is associated with and composes part of the A. nitens microbiota. This is the first report of Y. massiliensis in an insect host.
内共生细菌会影响生物参数，并降低天敌对目标昆虫的控制效果。本研究的目的是鉴定出平腹小蜂（Anaphes nitens）（膜翅目：缨小蜂科）中的内共生细菌，平腹小蜂是用于防治栗实象（Gonipterus platensis）（鞘翅目：象甲科）的主要天敌。从六个平腹小蜂种群中提取基因组 DNA，并用引物进行聚合酶链式反应（PCR），以检测这种昆虫中的内共生细菌。对 PCR 产物进行扩增、测序，并与 GenBank 中细菌的序列进行比较，以确定其种类。所有平腹小蜂种群都有耶尔森氏菌（肠杆菌目：肠杆菌科）。这种细菌最初被描述为自由生活，它与平腹小蜂的微生物群有关，并构成其一部分。这是耶尔森氏菌在昆虫宿主中的首次报道。
